Bulk B12 Powder

What Is Bulk B12 Powder?

Bulk B12 Powder is red crystal powder. It is soluble in water, most stable in pH4.5~5.0 weak acid conditions, in strong acid (pH2) or alkaline solution is decomposed. Heat can be a certain degree of destruction, but the loss of rapid high temperature disinfection is small. It is easily destroyed by strong light or ultraviolet light. Guanjie, as a manufacturer specializing in VB12 products for more than 10 years . High quality and competitive price, welcome to enquiry us.

 

COA:

Test items	Specifications	Results	Test methods
Characters	Dark red crystals or amorphous or crystallinered powder.	Complies	Visual method
Identification A	UV:The absorption spectrum exhibits maxima at 278+1nm361±1nm and 550+2nm	Complies	USP monograph
	A361nm/A278m:1.70~1.90 A361nm/A550nm:3.15~3.40	1.86 3.27
Identification B	Cobalt:Meets USP requirements	1.86 Complies	USP monograph
Ldentification C	HPLC:The retention time of the major peak of the	3.27	USP monograph
Loss on drying	≤10.0%	3.9%	USP monograph/USP
Assay	97.0%~102.0%	99.0%	USP monograph/USP<731>
Related substances	Total impurities≤3.0%	1.4%	USP monograph/USP
	7β,8β-Lactocyanocobalamin≤1.0%	0.6%
	34-Methylcyanocobalamin≤2.0%	0.2%
	8-Epi-cyanocobalamin≤1.0%	0.2%
	Any other unidentified impurity and50-Carboxycyanocobalamin、32-Carboxycyanocobalamin≤0.5%	0.2%
Acetone	≤5000ppm	116ppm	In house/(GC)
The total aerobic microbial count	≤1000 cfu/g	2.8x10²cfu/g	ChP2020<1105>
The total combined yeasts/mould count	≤100 cfu/g	<10cfu/g	ChP2020<1105>
Conclusion :	The product complies with the specification of USP 43 and in house standard.
Product Usage:	Not For Drug Use

 

Properties:
◆ Vitamin B12 (vitamin B12), also known as cobalamin, is one of the cobalt-containing B vitamins;.
◆ It is easily decomposed in strong acid or alkaline solution with pH less than 3 or more than 8, and easily destroyed by strong light or ultraviolet rays.
◆ Vitamin B12 has maximum absorption in the ultraviolet region at wavelengths. The absorption peak at 361nm has fewer interfering factors, so it is usually measured at 361nm.

 

Vitamin determination methods:
Include chemical analysis, colorimetry, spectrophotometry (ultraviolet and fluorescence), chromatography, chromatography (gas chromatography and high performance liquid chromatography) and microbiological methods.

 

Content Determination Methods:
◆Methods for the determination of vitamin B12 include microbiological method, colorimetric method, UV spectrophotometry, ion exchange chromatography, atomic absorption spectrophotometry and high performance liquid chromatography. UV spectrophotometry is the more common method.
◆Take the determination of Bulk B12 Powder content in vitamin B12 injection as an example to introduce the method of determining vitamin B12 content by UV spectrophotometry.

 

Determination by ultraviolet spectrophotometry

Determination principle
Vitamin B12 has maximum absorption at 278nm (nm), 361nm and 550nm in the ultraviolet region. The absorption peak at 361nm has few interfering factors, so 361nm is usually used as the wavelength of measurement, the absorbance of the solution to be tested is measured, and the content is calculated by the absorbance.

Instruments and reagents
1. Main instrumentation
◆Ultraviolet-visible spectrophotometer
◆ Volumetric flask 10ml
◆ Pipette

2. Reagents
◆ 0.1g/L vitamin B12 aqueous solution
◆Vitamin B12 injection

 

3. Procedure
◆ Accurately suck 0.50mL bulk B12 powder injection in a 10mL volumetric flask, add distilled water to the scale line, shake well to get the sample dilution.
◆ Turn on the UV-Vis spectrophotometer, preheat for 30min.
◆ Put the sample dilution in 1cm quartz cuvette, use distilled water as blank, and measure the absorbance at 361nm.
◆ Calculate the percentage of the labeled amount of vitamin B12 according to the absorption coefficient of C63H88CoN14O14P.

 

4. Recording and calculation of results

Calculation formula:
◆ VB12----the content of vitamin B12 in the sample, mg/mL
◆ A Absorbance of the solution to be tested;
◆ A0 The corrected value of the cuvette (absorbance);
◆ D Dilution of the test sample;
◆ W Sample mass (labeled amount of VB12 injection), mg/mL

 

Physiological function
Vitamin B12 exists in the body as two coenzymes, methyl B12 (methylcobalamin) and coenzyme B12 (5-deoxyadenosylcobalamin) and participates in biochemical reactions.
◆ Coenzyme for methyltransferases
Cofactors of methyltransferases are involved in the in vivo synthesis of methionine and thymine, thereby promoting protein and nucleic acid biosynthesis. For instance, methyl B12, as a cofactor of methionine synthase, gets methyl from 5-methyltetrahydrofolate and afterward goes to supply homocysteine, which is incorporated by methionine synthase.

◆ Involved in methylmalonic acid-succinic acid isomerization process
During metabolism in vivo, the reaction from methylmalonyl coenzyme A to succinyl coenzyme A requires the participation of coenzyme B12. When vitamin B12 powder bulk deficiency, this reaction can not be carried out, resulting in the accumulation of methylmalonic acid in the serum and increased urinary methylmalonic acid excretion.

 

Dangers of deficiency and overdose
Lack of vitamin B12 protein powder is entirely expected, and its wellbeing impacts, particularly in the old, are acquiring consideration. Vegans, babies and youngsters whose moms are veggie lovers, and the old are at high gamble for lack of Bulk B12.
Inadequate dietary intake; low gastric acid due to various factors; insufficient pancreatic protease secretion; ileal disease and reduced synthesis of serum holotranscobalamin II transport proteins can lead to reduced absorption of vitamin B12, which in turn can lead to vitamin B12 deficiency. A few examinations have shown that intrinsic vitamin B12 transport and digestion irregularities brought about via autosomal latent hereditary sicknesses can likewise prompt lack of b12 energy powder.

◆ Megaloblastic anemia
Vitamin B12 is associated with nucleic corrosive digestion in cells and is important for hematopoietic cycles. At the point when vitamin B12 is lacking, the response of demethylation of 5-methyltetrahydrofolate to tetrahydrofolate can't occur, which thusly prompts the deficient arrangement of 5,10-methylenetetrahydrofolate essential for the combination of thymine, bringing about the disability of DNA blend in erythrocytes, and actuating megaloblastic weakness.

◆ Nervous system damage
Vitamin B12 deficiency can cause patchy or diffuse nerve demyelination, this progressive neuropathy starts from peripheral nerves, gradually develops to the center and involves the spinal cord and the brain, forming a subacute compound degeneration, and neurological symptoms such as depression, memory loss and tremor of the limbs appear.
It has been recommended that vitamin B12-incited neurological harm is because of hindered union of methionine and S-adenosylmethionine because of methylvitamin B12 lack, and that the methyl communicating movement is viewed as the reason for typical working of the sensory system.

 

◆ Causes hyperhomocysteinemia
Lack of Bulk B12 Powder can cause hyperhomocysteinemia similarly as folic corrosive inadequacy. Lack of vitamin B12 prompts a development of homocysteine in the circulatory system since it can't be changed over completely to methionine. Hyperhomocysteinemia isn't just a gamble factor for cardiovascular sickness, yet can likewise cause neurological harm through poisonous impacts on synapses.
A few examinations have shown that lack of b 12 powder might be related with the improvement of infections like Alzheimer's illness, misery, maturing and mental deterioration or the seriousness of the sickness, yet this isn't yet indisputable.

 

Certifications:

Halal,ISO9001,PAHS Free,NON-GMO,KOSHER,SC

Delivery term:

DHL, FEDEX, Air freight,Sea freight

 

Where to buy Vitamin B12 (VB12) ?

When looking to purchase Bulk B12 Powder Vitamin B12 powder, you can choose Guanjie. Guanjie, as a manufacturer specializing in VB12 products for more than 20 years . With our own factory and dedicated R&D center, we have demonstrated a commitment to producing VB12 products. Our products pass Halal, ISO, PAHS Free, NON-GMO, KOSHER, and other certificates. For any requests in regards to our products, reach us at info@gybiotech.com.

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