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Raw Material Indole-3-Carbinol

Name: China Raw Material Indole-3-Carbinol Manufacturers Suppliers Factory - Wholesale Service - Guanjie Biotech
Brand: Guanjie Biotech
SKU: 172888572

Specification: 98%, 99% HPLC
Appearance: White crystalline powder
CAS No.:700-06-1
EINECS No.: 211-836-2
MOQ: 1kg
Delivery: Shipped in Same day from stocks
Certifications: Halal,ISO9001,PAHS Free,NON-GMO,KOSHER,SC
Payment term: L/C, D/A, D/P, T/T
Delivery term: DHL, Sea freight, FEDEX,Air freight

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Stability Data

Description

Raw Material Indole-3-Carbinol Supplier:

Guanjie Biotech is a professional Raw Material Indole-3-Carbinol supplier with 20 years of experience in researching, producing, and supplying high-quality natural ingredients. We provide bulk Indole-3-Carbinol powder with stable supply, sufficient inventory, factory direct prices, and flexible order solutions for global customers. Our Indole-3-Carbinol features high purity, excellent quality, and strong application value. As an important plant-derived compound, it is widely used in the pharmaceutical industry as a pharmaceutical intermediate. Raw Material Indole-3-Carbinol powder is also applied in dietary supplements, health care products, and other functional ingredient formulations. With strict quality control and advanced production technology, Guanjie Biotech ensures reliable products and competitive advantages for customers. Bulk Indole-3-Carbinol purchasing customers can enjoy more favorable prices, professional service, and customized solutions. Welcome to enquire with us at info@gybiotech.com.

I3C powder

COA

Items	Specifications	Result	Test Method
Appearance	Off-white to white crystalline powder	Conform	Visual
Odor, Taste	Characteristic	Conform	Sensory
Particle Size	98% pass 80mesh	Conform	GB/T 5507-2008
Content	≥99.0%	99.06%	HPLC
Residue Ignition	≤0.1%	0.06%	GB/T 9741-2008
Loss on drying	≤1.0%	0.59%	GB 5009.3-2016
Melting Point	92 -99℃	96.5℃	GB/T 21781-2008
Heavy Metals	≤10 ppm	Conform	GB 5009.74-2014
Pb	≤2 ppm	Conform	GB 5009.12-2017
As	≤2 ppm	Conform	GB/T 5009.11-2014
Hg	≤0.5 ppm	Conform	GB/T 5009.17-2014
Cd	≤0.5ppm	Conform	GB 5009.15-2014
Solvent Residue	No toluene or benzene	Absent	GB 5009.262-2016
Total plate count	≤1000 cfu/g	Conform	GB 4789.2－2016
Mold&Yeast	≤100 cfu/g	Conform	GB 4789.15－2016
E. Coli	Absent	Absent	GB 4789.38-2012
Salmonella	Absent	Absent	GB 4789.31-2016
Staphylococcus aureus	Absent	Absent	GB 4789.10-2016

How Is Indole-3-Carbinol Produced?

indole-3 flowchart

Synthesis Route

Raw materials: indole, phosphorus oxychloride and N, N-dimethylformamide

1) By the Vilsmeier-Haack reaction with a yield of 87.1%.

2) Toluene-water reaction system.

◆ Reducing agent: potassium borohydride.

◆ Temperature: 50～60℃.

◆ The reaction conditions were mild, and the reaction, extraction, and recrystallization were completed at one time, and the yield was 69.0%.

Synthesis process of Raw Material indole-3 -Carbinol

◆ Add 8.7kg indole-3-carbaldehyde, 145kg toluene, 6.48kg potassium borohydride, 48kg distilled water to the 300L enamel reaction kettle, heat, maintain the reaction temperature at 50～60 ℃ for 5h, cool to 15～20 ℃, add 50kg distilled water for treatment, centrifugal filtration, washing with distilled water, and drying to obtain 6.09 off-white flaky crystals with a yield of 69.0% and a melting point of 98 to 100 °C (documents 99 to 100 °C [10]).

◆ Elemental analysis, experimental value (theoretical value)/%

C73.58 (73.45), H6.20 (6.16), N9.42 (9.52). IR(KBr), γ/cm-1:3 380, 3052, 2927, 2865, 1543, 1455, 1420, 1356. 1H-NMR (500 MHz, DMSO-d6), δ: 4.67 (d, J = 5.3 Hz, 2H, CH2), 4.71 (dd, J = 6.0, 4.7 Hz, 1H, OH), 6.96–7.63 (m, 5H, ArH), 10.83 (s, 1H, NH).

Reference: [1] Shen Xinan. Synthesis of indole-3-methanol [J]. Modern Chemical Industry, 2008, (06): 43-45.

The effect on laryngeal carcinoma:

Raw Material Indole-3-Carbinol is a natural compound derived from cruciferous vegetables that has attracted attention due to its potential anti-tumor activities. Studies have investigated its effects on human laryngeal carcinoma cells, especially the Hep-2 cell line, to explore its possible mechanisms in cancer cell growth inhibition and apoptosis induction.

◆In the experiment, Hep-2 cells were divided into an experimental group and a control group. Different concentrations of indole-3-carbinol were added to the experimental groups, while the control group received no treatment. After 48 hours of treatment, researchers evaluated the effects of indole-3-carbinol on cell proliferation, apoptosis, and Livin protein expression.

The experimental results showed that the apoptosis rates of Hep-2 cells in different treatment groups were (3.95±0.68)%, (8.54±1.35)%, (21.28±2.32)%, and (38.65±4.85)%, respectively. The apoptosis rate of the control group was (4.26±0.58)%. These results demonstrated that Raw Material Indole-3-Carbinol significantly increased the apoptosis rate of Hep-2 cells in a concentration-dependent manner.

◆Further experiments indicated that indole-3-carbinol inhibited the proliferation of Hep-2 cells and promoted apoptosis. The anti-cancer effect was closely associated with the regulation of Livin protein expression. Livin is a member of the inhibitor of apoptosis protein (IAP) family and plays an important role in preventing programmed cell death. The study found that indole-3-carbinol reduced Livin protein expression as its concentration increased.

◆The decreased expression of Livin suggested a negative correlation between Livin protein levels and the apoptosis rate of Hep-2 cells after indole-3-carbinol treatment. This indicates that Raw Material Indole-3-Carbinol may induce laryngeal carcinoma cell apoptosis by inhibiting Livin protein expression, thereby suppressing cancer cell survival and growth.

In conclusion, Livin protein may play an important role in the apoptosis of human laryngeal carcinoma cells induced by indole-3-carbinol. As a natural compound with high efficiency, low toxicity, and potential anti-tumor properties, Raw Material Indole-3-Carbinol may become a promising candidate for cancer prevention and supportive treatment research. It mainly targets tumor cells while showing low cytotoxicity toward normal non-tumor cells. However, further studies are required to clarify the molecular mechanisms of indole-3-carbinol in laryngeal cancer and provide more scientific evidence for future clinical applications.

Reference: Chinese Journal of Clinical Pharmacology, 2015, (11): 932-934+959.

Indole powder cancer

What Is Indole-3-Carbinol Used For?

Raw Material Indole-3-Carbinol is a natural bioactive compound mainly found in cruciferous vegetables, such as broccoli, cabbage, cauliflower, and Brussels sprouts. It is widely used as a functional ingredient in the food, dietary supplement, pharmaceutical, and feed industries. As a professional China Indole-3-Carbinol supplier, Guanjie Biotech provides high-quality I3C powder for different applications, including food grade, feed grade, and pharmaceutical grade products.

Raw Material Indole-3-Carbinol is commonly studied for its potential biological activities and health applications. In scientific research, Indole-3-Carbinol has been used as a raw material to develop advanced delivery systems. For example, I3C has been encapsulated with poly-lactic-co-glycolic acid (PLGA) to evaluate its effects on different cancer cell models, including breast adenocarcinoma epithelial cells (MCF7), colon adenocarcinoma epithelial cells (Caco-2), and prostate cancer epithelial cells (PC3). These in vitro studies have investigated the potential anticancer activity and cellular response mechanisms of I3C.

In addition, Raw Material Indole-3-Carbinol powder bulk is often used as a cytochrome P4501A (CYP1A) enzyme inducer in biochemical research. Its ability to regulate enzyme activity makes it an important research compound for studying metabolic pathways, hormone metabolism, and cellular signaling processes.

What are The Biochemical and Physiological Effects of Indole-3-Carbinol?

Raw Material Indole-3-Carbinol has attracted significant attention due to its potential role in regulating carcinogenesis-related pathways. Research suggests that I3C may help suppress carcinogenesis during the early initiation stage. Animal studies have shown that I3C can inhibit the development of certain cancers when administered before tumor formation. However, its effects may vary depending on the timing and conditions of administration. Some studies indicate that treatment after tumor development may produce different biological responses.

● One of the important mechanisms of Indole-3-Carbinol involves activation of the aryl hydrocarbon receptor (AhR).

Through AhR signaling, I3C can influence gene expression related to cell growth, differentiation, and detoxification processes. It has been reported that I3C may induce G1 cell cycle arrest and promote apoptosis in certain cancer cell lines, making it a valuable compound for cancer-related research.

● Another important biological function of I3C is its influence on estrogen metabolism.

Pure Indole-3-Carbinol powder can stimulate cytochrome P450 enzymes, especially CYP1 family enzymes, which are involved in estrogen metabolism pathways. By promoting the conversion of estrogen metabolites, I3C has been investigated for its potential role in maintaining balanced hormone metabolism.

Due to these biological activities, Raw Material Indole-3-Carbinol is considered a promising research ingredient for applications related to breast cancer, prostate cancer, colon cancer, and leukemia studies. It is widely used in nutritional supplements designed to support hormone balance and overall wellness.

For industrial applications, high-quality Indole-3-Carbinol powder requires strict quality control, accurate testing methods, and stable production processes. Suppliers usually provide different grades according to customer requirements, including nutritional supplement grade, pharmaceutical research grade, and feed application grade. With its natural origin, broad application potential, and extensive scientific research background, Raw Material Indole-3-Carbinol continues to be an important ingredient in the nutraceutical and pharmaceutical industries.