Raw Material Indole-3-Carbinol

Introduction:

Indole-3-carbinol be found in a large number of plants. Such as broccoli, cabbage, cauliflower, purple cabbage, kale, etc.And it is important for the human body. With the highest content, high nutritional value, and medicinal value, in the pharmaceutical industry as a pharmaceutical intermediate, raw Material Indole-3-Carbinol can also be used in dietary supplements, intermediates in health care products and other fields.

Guan jie specialized in plant extracts for 17 years, and now produces indole supplements, with sufficient inventory, factory direct sales, affordable prices, and more benefits and discounts that can be enjoyed by ordering in large quantities.

COA

Items	Specifications	Result	Test Method
Appearance	Off white to white crystalline powder	Conform	Visual
Odor, Taste	Characteristic	Conform	Sensory
Particle Size	98% pass 80mesh	Conform	GB/T 5507-2008
Content	≥99.0%	99.06%	HPLC
Residue Ignition	≤0.1%	0.06%	GB/T 9741-2008
Loss on drying	≤1.0%	0.59%	GB 5009.3-2016
Melting Point	92 -99℃	96.5℃	GB/T 21781-2008
Heavy Metals	≤10 ppm	Conform	GB 5009.74-2014
Pb	≤2 ppm	Conform	GB 5009.12-2017
As	≤2 ppm	Conform	GB/T 5009.11-2014
Hg	≤0.5 ppm	Conform	GB/T 5009.17-2014
Cd	≤0.5ppm	Conform	GB 5009.15-2014
Solvent Residue	No toluene or benzene	Absent	GB 5009.262—2016
Total plate count	≤1000 cfu/g	Conform	GB 4789.2－2016
Mold&Yeast	≤100 cfu/g	Conform	GB 4789.15－2016
E. Coli	Absent	Absent	GB 4789.38-2012
Salmonella	Absent	Absent	GB 4789.31-2016
Staphylococcus aureus	Absent	Absent	GB 4789.10-2016

Flowchart:

Synthesis Route

Raw materials: indole, phosphorus oxychloride and N,N-dimethylformamide

1) By Vilsmeier-Haack reaction with a yield of 87.1%.

2) Toluene-water reaction system.

◆ Reducin agent: potassium borohydride.

◆ Temperature: 50～60℃.

◆ The reaction conditions were mild, and the reaction, extraction and recrystallization were completed at one time, and the yield was 69.0% .

◆ Add 8.7kg indole-3-carbaldehyde, 145kg toluene, 6.48kg potassium borohydride, 48kg distilled water to the 300L enamel reaction kettle, heat, maintain the reaction temperature at 50～60 ℃ for 5h, cool to 15～20 ℃, add 50kg distilled water for treatment , centrifugal filtration, washing with distilled water, and drying to obtain 6.09 off-white flaky crystals with a yield of 69.0% and a melting point of 98 to 100 °C (documents 99 to 100 °C [10]).

◆ Elemental analysis, experimental value (theoretical value)/%

C73.58 (73.45), H6.20 (6.16), N9.42 (9.52). IR(KBr), γ/cm-1:3 380, 3052, 2927, 2865, 1543, 1455, 1420, 1356. 1H-NMR (500MHz, DMSO-d6), δ: 4.67 (d, J=5.3Hz, 2H, CH2), 4.71 (dd, J=6.0, 4.7 Hz, 1H, OH), 6.96-7.63 (m, 5H, ArH), 10.83 (s, 1H, NH).

Reference: [1] Shen Xinan. Synthesis of indole-3-methanol [J]. Modern Chemical Industry, 2008, (06): 43-45.

The effect on laryngeal carcinoma:

◆ Experimental group and control group treatment

◆ Experiment duration: 48 hours later.

◆ Experimental results: The apoptosis rates of Hep-2 cells were (3.95±0.68)%, (8.54±1.35)%, (21.28±2.32)%, (38.65±4.85)% and (4.26±0.58)%, respectively.

◆ Experiments showed

1)Indole-3-carbinol inhibited the proliferation of Hep-2 in a concentration-dependent manner, induced apoptosis and the expression of Livin protein.

2) Indole-3-carbinol can inhibit the proliferation of Hep-2 cells, and its induction of apoptosis may be related to the inhibition of Livin protein expression.

3) With the increase of indole-3-carbinol concentration, Livin expression of Chemicalbook gradually decreased, suggesting that Livin protein expression was negatively correlated with the apoptosis rate of human laryngeal carcinoma cell line Hep-2 after indole-3-carbinol treatment.

◆ Conclusion: Livin protein may play a certain role in the apoptosis of human laryngeal carcinoma cells induced by Raw Material Indole-3-Carbinol. Indole-3-carbinol can only induce apoptosis of cancer cells, is safe for non-tumor cells, and has no cytotoxicity.

Because of its high-efficiency, non-toxic and natural anti-tumor properties, indole-3-carbinol can be one of the candidates for cancer prevention and treatment. The molecular mechanism of indole-3-carbinol inhibiting laryngeal cancer cells still needs further study in order to provide a theoretical basis for future clinical drug research.

References:[2] Chinese Journal of Clinical Pharmacology, 2015, (11): 932-934+959.

Application of Indole Trimethanol

Raw Material Indole-3-Carbinol was used to encapsulate poly-lactic-co-glycolic acid (PLGA) to study its effect on breast adenocarcinoma epithelial cells (MCF7), colon adenocarcinoma epithelial cells (Caco2), prostate cancer epithelial cells (PC3) in vitro anticancer effect. It has also been used as a cytochrome P4501A (CYP1A) inducer.

Often used as food grade, feed grade and pharmaceutical grade.

Biochemical/Physiological Effects

Suppresses carcinogenesis at the initial stage. It has been shown to inhibit carcinogenesis in several animals, but it increases tumor incidence if administered in the post-onset stage. Found in cruciferous vegetables. Indole-3-carbinol Powder activates the aryl hydrocarbon receptor (AhR) to induce G1 cell cycle arrest and apoptosis. Therefore, it is a potential anticancer agent. Furthermore, it induces estradiol metabolism by stimulating cytochrome P450 enzymes. Therefore, I3C is considered to be an effective chemotherapeutic agent for various types of cancer, including breast, prostate, colon and leukemia.

Molecular structure data

◆ Molar Refractive Index: 44.97

◆ Molar volume (m3/mol): 115.6

◆ Isometric specific volume (90.2K): 324.2

◆ Surface tension (dyne/cm): 61.7

◆ Dielectric constant

◆ Dipole distance (10-24cm3)

◆ Polarizability: 17.83

Computational chemical data of Raw Material Indole-3-Carbinol

◆ Hydrophobic parameter calculation reference value (XlogP): 1.1

◆ Number of hydrogen bond donors: 2

◆ Number of hydrogen bond acceptors: 1

◆ Number of rotatable chemical bonds: 1

◆ Topological molecular polar surface area (TPSA): 36

◆ Number of heavy atoms: 11

◆ Surface charge: 0

◆ Complexity: 138

◆ Number of isotope atoms: 0

◆ Determine the number of atomic stereocenters: 0

◆ Uncertain number of atomic stereocenters: 0

◆ Determine the number of chemical bond stereo centers: 0

◆ Uncertain number of chemical bond stereocenters: 0

◆ Number of covalent bond units: 1

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Research Center:

Certification

Package:

1kg/Al.foil bag with transparent plastic bags inside

25kg/Paper drum with black plastic bag inside

Shipment:

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