Milk Thistle Extract Powder

Milk thistle extract powder Supplier:

Guanjie is a supplier of high-quality Milk Thistle bulk Extract Powder, which is made from the seeds of the plant. The extract is standardized to contain 80% Silymarin (measured by UV) and 30% Silybin (measured by HPLC).  The powder has a yellowish hue and a fine texture, making it easy to mix into drinks, smoothies, or food. The extract is also available in an 80 mesh size, which means it is finely ground and easy to absorb. High quality and competitive price, welcome to inquiry us.

Why Choose Guanjie?

● Independent factories and labs.

● Provide the third party testing.

● Have been on this field for 20 years.

● Have strong Collaborators:Northwestern University and Chinese Academy of Sciences research center. 

Brief Introduction:

Milk thistle extract powder is made from the dried fruit of Silybum marianum G., and commercial extracts are usually standardized to contain 80% of total silymarin. Milk thistle is an annual or biennial herb, native to southern Europe and North Africa, where it has been cultivated and used for thousands of years and is a folk medicinal plant. Milk thistle is widely used as a remedy for liver disease and cardiovascular disease. It is cultivated in North China and Northwest my country.

Certificate of Analysis

Flow Chart

Identifications

1: Microscopic identification

The epidermis of the pericarp wall contains almost colorless palisade cells, arranged at right angles to the surface. Tracheids with thick outer walls and longitudinal fissures. There are thickened ridges like granular thickened cell walls. The lower epidermis of the pericarp is composed of non-lignified parenchyma cells and constitutes a pigmented layer. Colorless cells and variable numbers of pigment cells are interlaced, so the appearance of the peel appears to be striped. 

The pericarp wall tissue is about 8 layers of cells thick, and there are parenchyma cells scattered along the longitudinal axis of the fruit. The innermost cells of the peel may be crushed and contain cigar-shaped or monoclinic calcium oxalate crystals. The seed coat is composed of yellow large palisade cells, which contain a long and narrow tracheid, slightly swollen at the end, and the cell wall has obvious stratification. 

There are special punctate cells under the epidermis of the seed coat, and the lignified cell membrane has obvious, confined ridges or thickening (reticular cells). There is a layer of cells near it, the cell wall is thick, slightly swollen and lipophilic. Embryos consist of parenchyma cells with small glands, massive crystals and fat droplets visible.

2:Thin layer identification

Test solution: According to the operation of "Sample Solution Preparation" under 4 items of 【Analysis Method】. Standard solution: 1.0 mg/ml silibinin standard in methanol. 

Developing solvent: freshly prepared mixture of chloroform, acetone, and anhydrous formic acid (75:16.5:8.5). Detection: The thin plate was dried in a cold air flow for 30 minutes, sprayed with 1:100 diphenylboron ethoxyamine methanol solution, left to dry. Then sprayed with 5% polyethylene glycol 4000.

After 1 hour, it was exposed to a long-wave UV lamp Under observation, the test solution has a strong green-blue fluorescent band with an Rf value of about 0.5 (due to the presence of silibinin), a gray-blue point with an Rf value of about 0.4, which corresponds to a point in the standard solution . 

The test solution also showed other bands: a strong green-blue band with an Rf value of about 0.25 (subsilymarin), a red-orange-yellow band with an Rf value of about 0.3 (docetaxel).

Quality Inspection

Thin layer identification or DAB10 compliance:

● Other organic matter: n

Not more than 2.0%.

● Weight loss on drying: 

1.0g of milk thistle powder was dried at 105°C for 2 hours, and the weight loss did not exceed 12.0%.

● Total ash: 

1.0g milk thistle powder measured total ash not more than 8.0%.

● Heavy metals: 

Not more than 0.001%; or heavy metals: Pb≤5ppm, Cd≤0.2ppm, Hg≤0.1ppm.

● Insecticide Residues: 

Complies with PHmV (1989).

● Microbial limit:t

The total number of bacteria does not exceed 10000/g; the total number of mold and yeast does not exceed 100/g; Salmonella, Escherichia coli, Staphylococcus aureus meet the regulations; or DAB10: Ⅷ. N7, category 4a.

● The content of silymarin: 

This product is not less than 1.0% (DAB10) calculated as Silibin (C25H27O10). Note: The US Pharmacopoeia stipulates that it should not be less than 2.0%.O10). 

● Note: 

The US Pharmacopoeia stipulates that it should not be less than 2.0%.

Analytical method

1. Spectrophotometric Determination of Silymarin Content

Accurately weigh an appropriate amount of this product powder, put it in a 50ml volumetric flask, add 40ml of absolute ethanol to dissolve, make up to volume, shake well, accurately draw 5ml into a 100ml volumetric flask, add absolute ethanol to the mark, shake well, and do a Blank control. Absorbance was measured at 288 nm with a UV spectrophotometer. Calculate the content as follows:

Silymarin content %= (calculated as silybin)×100%

A: The absorbance value of the sample; M: The sampling amount (g); V: The dilution factor of the sample; 470: The absorbance coefficient of silibinin.

Note: This method is derived from the local standard of Liaoning Province, and is suitable for the determination of the content of milk thistle extract with the value of HPLC method not less than 60%.

2. Spectrophotometric Determination of Silymarin

Precisely weigh about 250mg of the refined extract, put it in a 50ml volumetric flask, add an appropriate amount of methanol to dissolve, dilute to 50ml, shake well, and the solution is the solution to be tested.

Take 1ml of the test solution into a 10ml volumetric flask, add 2ml of 2,4-dinitrophenylhydrazine reagent, shake well, plug the stopper, heat at 50°C for 50 minutes, cool, and add potassium hydroxide methanol solution to the mark , Shake well and let stand for 2 minutes. Take 1ml of this solution into a centrifuge tube, add 20ml of methanol, shake well, and centrifuge. Pour the red-brown supernatant into a 50ml volumetric flask, add 20ml of methanol to the residue and centrifuge, combine the supernatant, and dilute to the mark with methanol.

Precisely draw 1ml of methanol and put it into a 10ml volumetric flask, and operate in the same way from "add 2ml of 2,4-dinitrophenylhydrazine reagent" as a blank control.

Taking the blank solution as the control, the absorbance A value of the measured concentration was measured at 490 nm, and the content of silymarin was calculated with E=537.

3. Determination of silymarin content by spectrophotometry (German Pharmacopoeia DAB-9 edition 1997)

● Preparation of standard solution: 

Precisely weigh 25 mg of standard silibinin, place it in a 5 ml volumetric flask, add methanol to dissolve and dilute to the mark to obtain a standard solution with a concentration of 5 mg/ml.

● Preparation of sample solution:  

Weigh 250mg of Milk thistle extract powder into a 50ml volumetric flask, dissolve and dilute to the mark with methanol, and set aside.

● Preparation of color developing solution: 

2,4-dinitrophenylhydrazine (2,4-dinitrophenylhydrazine) test solution: weigh 500mg of this reagent in a 50ml volumetric flask, add H2SO41ml, and dilute to the mark with methanol.

● 10% KOH solution: 

Weigh 10g KOH into a 100ml volumetric flask. Add 30ml of water and dilute to the mark with methanol.

Determination method: 

Take 1 ml Silymarin Powder of standard solution, sample solution and methanol respectively and put them in 10ml volumetric flasks, respectively add 2ml of 2,4-dinitrophenylhydrazine test solution, and react the three volumetric flasks in a water bath at 50-55°C. 50min, after rapid cooling with tap water, add KOH solution to the mark, draw 1 ml of the above three reaction solutions into a 10ml test tube with a stopper, add 10ml methanol, mix well, centrifuge for 10min, and pour the supernatant into a 50ml volume respectively. 

In the bottle, centrifuge the precipitate and add 10ml of methanol to mix, centrifuge, and then pour the supernatant into the same 50ml volumetric flask, and repeat once, add methanol to the mark in all three 50ml volumetric flasks. Shake well, and measure the absorbance value at 490nm with UV spectrophotometer.

The calculation of the above formula is based on the sampling operation and weighing strictly according to the above requirements. If the weighing cannot accurately meet the requirements in the text, it can be calculated according to the following formula:

A1: Sample absorbance value; A2: Standard sample absorbance value; W1: Sample sampling volume (g); W2: Standard sample sampling volume (g); V1: Sample dilution volume; V2: Standard dilution volume

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