Sodium Aesculin

Sodium Aesculin Suppliers:

Guanjie Biotech is a professional supplier of Aesculin. Sodium Aesculin is extracted from the fruit of horse chestnut. We offer various specifications. It is available as a white powder or white crystalline powder. Guanjie has been focusing on plant extracts for 20 years, accumulating significant expertise. We prioritize natural sources and deliver high-quality products at competitive prices. If you're interested in our offerings, please contact us at: info@gybiotech.com.

 

Why Choose Guanjie Biotech?

●Sodium Aescinate stands as our flagship product; we operate two production lines with an annual production capacity of 20 tons of Sodium Aescinate 98%.
● We currently serve over 50 customers from the USA, EU, Southeast Asia, and beyond.
● Our facility is equipped with various testing machines including HPLC, GC, AAS, melting point apparatus, specific optical rotation analyzer, bacterial detection equipment, pH meters, and ash content testers.

 

What Is Sodium Aesculin?

The primary components are Aescin Sodium A and Aescin Sodium B. It is a triterpene saponin containing a sester bond, extracted from the dried mature seeds of the horse chestnut plant, Aesculus hippocastanum. Clinically, it is utilized for treating cerebral edema, swelling due to trauma or surgery, and venous reflux disorders.

 

COA:

Item	Specification	Result	Method
Basic Product Information
Genus and Species	Aesculus hippocastanum L.	Conform	/
Part of the Plant	Seed	Conform	/
Country of Origin	China	Conform	/
Marker Compounds
Escin (C55H85NaO24)	97.5%~102%	97.8%	HPLC
Aescin A	25.5%~44.5%	38.4%
Aesculuside B	20.5%~34.5%	29.5%
Organoleptic Data
Appearance	White powder or crystalline powder	White crystalline powder	GJ-QCS-1008
Solubility	Easily soluble in water, slightly soluble in ethanol and extremely slightly soluble in dilute hydrochloric acid solution	Conform	/
Clarity and color of the solution	The solution should be clear and colorless	Conform	/
Odor	Characteristic	Conform	GB/T 5492-2008
Taste	Characteristic	Conform	GB/T 5492-2008
Rotation	-16.0-- -26.0	-18.9	/
Process Data
Method of Processing	Extraction	Conform	/
Solvent(s) Used	Grain alcohol/water	Conform	/
Excipient	None	Conform	/
Physical Characteristics
Loss on Drying	<5.0%	2.30%	GB/T 14769-1993
PH	5.2~6.8	6.2
Kali salt	≤0.20%	Conform
Arsenic salt	≤0.0002%	Conform
Heavy Metals
Total Heavy Metals	≤0.0015%	Conform	/
Microbiology
Total Plate Count	<100/g	Conform	/
Bacterial endotoxin	< 8EU/1mg	Conform	/

 

How To test Sodium Aesculin?

There are four kinds of target ingredients in the Sodium Aesculin product, Escin IA, Escin IB, Isoescin IA, Isoescin IB

The retention time of the target ingredient peak in the chromatogram of the assay preparation corresponds to that of the target ingredient peak in the chromatogram of the Standard preparation as obtained in assay.

ASSAY (TOTAL ISOFLAVONES CONTENT):

By high-performance liquid chromatography.

Chromatographic Conditions:

Instrument:	Shimadzu LC-9A Pumps, SPD 6A UV Detector
	SIL 6B Auto Injector and CR 7A Data
	Processor
Column:	NOVAPACK, C18 , 5μm, 150x4.6mm
Mobile phase :	CH3CN:0.4% Phosphoric Acid Water solution (30:8:62);adjust the PH to 2.1
Flow rate:	1.0 ml/minute
Detector:	UV-220 nm
Injection volume:	20 microliters

 

Standard Preparation

Working Standard Solution

Accurately weigh approximately 6 mg each of Escin IA, Escin IB, Isoescin IA, and Isoescin IB reference standards (collectively recorded as W1). Transfer the weighed standards into a clean 10 mL volumetric flask. Add an appropriate quantity of methanol to dissolve the contents completely, ensuring full solubilization through gentle mixing or sonication if necessary. After dissolution, dilute to volume with methanol and mix thoroughly to obtain a homogeneous working standard solution suitable for chromatographic analysis.

Sample Preparation

Accurately weigh 50 mg of the test Sodium Aesculin sample (recorded as W2) and transfer it into a previously dried 10 mL volumetric flask to avoid moisture interference. Add methanol to dissolve the sample completely. If required, sonicate briefly to facilitate dissolution and ensure uniform extraction of the analytes. After complete dissolution, dilute to the mark with methanol and mix well to produce a clear and uniform test solution for analysis.

Chromatographic Procedure

Inject equal volumes (20 µL) of both the standard preparation and the sample preparation separately into the chromatographic system. Each solution should be injected in triplicate to ensure analytical precision and reproducibility. Record the chromatograms obtained for each injection and carefully note the peak areas corresponding to the individual target components, namely Escin IA, Escin IB, Isoescin IA, and Isoescin IB. The average peak area values obtained from replicate injections should be used for quantitative calculations.

Calculation

The content of each target component in the Sodium Aesculin sample is calculated using the following formula:

One target ingredient (%) = (At × W1 × Ps × 100%) / (As × W2)

Where:

At = Peak area of the specific target ingredient in the test preparation chromatogram

As = Peak area of the corresponding ingredient in the standard preparation chromatogram

W1 = Weight of the reference standard used (mg)

W2 = Weight of the test sample (mg)

Ps = Purity or content of the corresponding reference standard (%)

Total Escins Content

The total escins content, expressed as percentage weight by weight (% w/w), is calculated as the sum of all quantified escin components:

Total Escins Content (% w/w) = Escin IA (% w/w) + Escin IB (% w/w) + Isoescin IA (% w/w) + Isoescin IB (% w/w)

This calculation represents the overall escin concentration present in the analyzed sample.

 

 

What Does Sodium Aesculin Do?

● Generally regarded as safe under proper use
Sodium aesculin is considered safe when used according to recommended concentrations and application guidelines. It is commonly applied in laboratory media, cosmetic formulations, and research environments where controlled usage standards are followed.

● Derived from natural plant compounds
Sodium aesculin is the sodium salt of aesculin, a naturally occurring coumarin glycoside found in plants such as horse chestnut. Its botanical origin and long history of scientific use contribute to its established safety profile.

● Low toxicity characteristics
Available toxicological data suggest that sodium aesculin has low acute toxicity when handled appropriately. Under standard industrial and laboratory conditions, it demonstrates good compatibility with biological systems.

● Safe for topical and laboratory applications
When incorporated into properly formulated products, sodium aesculin typically shows minimal irritation potential. This makes it suitable for topical preparations and microbiological diagnostic media when concentration limits are respected.

● Quality and purity are essential factors
Safety depends heavily on manufacturing quality, purity levels, and compliance with technical specifications. High-quality raw materials produced under standardized conditions help minimize contamination risks and ensure consistent performance.

● Avoid excessive or unapproved oral intake
Although safe in controlled applications, excessive ingestion or use outside approved purposes may lead to adverse reactions. Oral consumption should only occur where safety evaluations and regulatory approvals exist.

● Precautions for sensitive groups
Pregnant individuals, children, and those with underlying medical conditions should exercise caution and consult qualified professionals before exposure.

 

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