Indole-3-carbinol Powder

Bulk Indole-3-Carbinol Powder Supplier | Guanjie Biotech

Guanjie Biotech is a professional indole-3-carbinol powder manufacturer and bulk I3C powder supplier with over 20 years of industry experience. We operate a raw material synthesis workshop and follow strict quality standards to ensure consistent product quality. Our indole-3-carbinol powder appears as an off-white to white crystalline powder. We focus on product quality and stable performance. Our 30-member sales team in China provides professional customer support, while our offices and warehouses in the United States and the United Kingdom help serve international customers. For businesses seeking a reliable I3C ingredient supplier for bulk purchasing and long-term cooperation, Guanjie Biotech is a dependable partner.

 

Indole-3-carbinol (I3C) is formed from glucobrassicin, a glucosinolate naturally found in cruciferous vegetables. Indole-3-carbinol powder is associated with plant sources such as broccoli, cabbage, cauliflower, Brussels sprouts, collard greens, and kale. It is also available as a dietary supplement ingredient. For proper storage, keep bulk indole-3-carbinol powder in a cool, dry, well-sealed container, protected from strong light and heat. Follow the product specification and supplier's recommendations, including the specified storage temperature of 0–8°C, to help maintain product quality.

 

Product quality steps:

◆ We will prepare Indole-3-carbinol Powder samples for approval;

◆ After getting approved, our team will set up a process craft, and form a inside drawing to follow it;

◆ During production, we have FQC, IQC, IPQC and OQC to control the quality.

 

COA

Product Name: pure indole 3 carbinol
Items	Standard	Results	Test Method
Appearance	Off-white to white crystalline powder	Conform	Visual
Odor, Taste	Characteristic	Conform	Sensory
Mesh size	98%pass 80mesh	Conform	GB/T 5507-2008
Content	≥99.0%	99.1%	HPLC
Residue Ignition	≤0.1%	0.06%	GB/T 9741-2008
Loss on drying	≤1.0%	0.6%	GB 5009.3-2016
Melting Point	92 -99℃	96.5℃	GB/T 21781-2008
Heavy Metals	≤10 ppm	Conform	GB 5009.74-2014
Pb	≤2 ppm	Conform	GB 5009.12-2017
As	≤2 ppm	Conform	GB/T 5009.11-2014
Hg	≤0.5ppm	Conform	GB/T 5009.17-2014
Cd	≤0.5ppm	Conform	GB 5009.15-2014
Solvent Residue	No toluene or benzene	Absent	GB 5009.262-2016
Total plate count	≤1000 cfu/g	Conform	GB 4789.2－2016
Mold&Yeast	≤ 100cfu/g	Conform	GB 4789.15－2016
E.coli	Absent	Absent	GB 4789.38-2012
Salmonella	Absent	Absent	GB 4789.31-2016
Staphylococcus aureus	Absent	Absent	GB 4789.10-2016

How To Produce Indole-3-carbinol Powder?

 

Indole-3-carbinol Test SOP

(Standard Operating Procedure)

1. Objective:

This standard operating procedure (SOP) establishes testing requirements for indole-3-carbinol (I3C) powder to ensure accurate and consistent quality control results. It applies to finished indole-3-carbinol powder products.

2. Responsibilities and Standards

The Quality Control (QC) department is responsible for testing according to customer specifications and approved procedures.

3. Properties and Solubility

Indole-3-carbinol powder is a white to almost-white crystalline powder. It is practically insoluble in water, soluble in 96% ethanol, and slightly soluble in dichloromethane.

4. Loss on Drying

The loss on drying specification is not more than 1.0%. Weigh approximately 100 mg of sample in a constant-weight, flat weighing flask. Dry at 45°C for two hours, cool to room temperature in a desiccator, and weigh accurately. Repeat the drying cycle and weighing. The difference between consecutive measurements should be less than 0.3 mg.

5. Indole-3-Carbinol Powder Purity

The content specification is 98%–102% on a dry basis. Use an analytical balance, HPLC system, HPLC-grade methanol, purified water, and analytical-grade triethylamine.

6. HPLC Testing Conditions

Use an Xtimate C18 column (4.6 × 250 mm, 5 μm), methanol–water (60:40) adjusted to pH 8.0 with triethylamine, a detection wavelength of 254 nm, a column temperature of 30°C, a flow rate of 1.0 mL/min, and an injection volume of 20 μL.

Dissolve 10 mg of indole-3-carbinol reference standard in a 25 mL volumetric flask. Prepare test samples similarly in brown volumetric flasks using methanol, and sonicate for five minutes at room temperature before injection. Verify reference-solution repeatability and sample calculations using the approved analytical method.

 

7. Data recording and calculation: external standard method

As --Sample peak area

WS --Sample weighed

AR --Standard peak area

WR --Standard product weighed

A--Sample loss on drying

CR --Standard content

 

8. Operation Cautions:

1. This Indole-3-carbinol Powder product is sensitive to light and heat, with the influence of temperature and time. Experiments must be as fast as possible, and should avoid light and air.

2. The goods should be stored in a sealed container, protected from light, keep under -10 ° C or less.

HPLC digital fingerprint

 

Stability Test Methods

● Introduction:

The purpose of stability testing is to provide evidence on how the quality of Indole-3-carbinol Powder varies with time under the influences of temperature and humidity and to

establish recommended storage conditions, retest periods, and shelf life.

● Responsibility:

QA/QC Director is responsible for the stability study program and is also responsible for the stability samples analyzed as well as the maintenance of the program records.

● Procedure and Design of Stability Studies:

● Long Term Studies:

The samples of Indole-3-Carbinol are closed tightly in polyethylene bags, sealed in PE bags, and packaged in aluminum containers. They are stored under long-term conditions of 252℃ temperature and 60±5% humidity conditions according to ICH/FDA guidelines. The three consecutive process validation batches and one batch every year are sampled and placed on long-term stability testing. After the initial analysis, these samples will be analyzed at intervals of 3, 6, 9, 12 and 15 months. The results to date demonstrate that Indole-3-Carbinol is stable at 25±2℃temperature and 60±5% humidity conditions.

 

Accelerated Studies:

The samples of pure Indole-3-Carbinol are placed in polyethylene bags, sealed in PE bags, packaged in aluminum containers and stored at 40±2℃ and 75±5% humidity conditions. The three consecutive batches are taken for testing. After the initial analysis, these samples are analyzed at 1, 2, 3 and 6 months according to ICH Guidelines. The results to date demonstrate that Indole-3-carbinol Powder is stable at 40±2℃ temperature and 75±5% humidity conditions.

 

Accelerated Studies:

The samples of Indole-3-carbinol Powder are placed in polyethylene bags, sealed in PE bags, packaged in aluminum containers and stored at 40±2℃ and 75±5% humidity conditions. The three consecutive batches are taken for testing. After the initial analysis, these samples are analyzed at 1, 2, 3 and 6 months according to ICH Guidelines. The results to date demonstrate that Indole-3-Carbinol is stable at 40±2℃ temperature and 75±5% humidity conditions.

 

Degradation Study:
The purpose of the degradation study is to determine if the HPLC method used by our facility for product release and stability studies will detect impurities and as a result is stability indicating. Three lots on stability, IC140910, IC140926, IC140930 were chosen and samples from these lots were subjected to:
a. Sodium Hydroxide, 0.00001 N
b. Hydrochloric Acid, 0.04 N
c. Hydrogen Peroxide, 10%
d. Heat at 60℃
e. Ultraviolet light

What is Indole-3-carbinol powder Used For?

◆ Medicine Field:

◆ Health Care Field:

As an antioxidant and anti-aging agent, it can scavenge free radicals, promote cell metabolism, prevent aging, and improve skincare. It can also reduce senile heart disease, hyperglycemia, and Alzheimer's disease, and prolong life.

◆ Cosmetics Field:

Indole-3-carbinol Powder can be added to cosmetics, skincare, and anti-aging.

 

◆ Regulation of estrogen

Indole-3-carbinol powder inhibits estrogen synthesis, blocks estrogen-related pathways, and inhibits estrogen-induced cell proliferation. Because of the existence of these mechanisms, indole-3-methanol is thought to be used to regulate diseases associated with hyperestrogenism.

◆ Indole-3-carbinol powder is used for the treatment of fibromyalgia, virus-induced intralaryngeal tumor (laryngeal papillomatosis), virus-induced respiratory tract tumor (respiratory papillomatosis), abnormal growth of cervical cells (cervical dysplasia), systemic erythema Lupus (SLE).

 

FAQs:

Q1. What is the difference between natural indole-3-carbinol and synthetic I3C?

Natural indole-3-carbinol (I3C) is formed from glucobrassicin, a naturally occurring glucosinolate found in cruciferous vegetables such as broccoli, cabbage, and Brussels sprouts. Synthetic I3C is produced through chemical synthesis. Both forms contain the same target chemical compound, but their manufacturing processes, impurity profiles, and quality specifications may differ. Buyers should evaluate purity, identity, and analytical documentation when selecting I3C raw materials.

Q2. Is natural indole-3-carbinol better than synthetic I3C?

Natural I3C is not automatically superior to synthetic I3C. The suitability of either ingredient depends on its purity, manufacturing controls, impurity profile, stability, and intended application. For dietary supplement and nutraceutical manufacturers, a well-characterized ingredient with consistent specifications and reliable analytical testing is generally more important than the natural or synthetic label alone.

Q3. What are the main sources of natural indole-3-carbinol?

Natural indole-3-carbinol originates from glucobrassicin in cruciferous vegetables, including broccoli, cabbage, cauliflower, kale, and Brussels sprouts. When plant tissue is cut or crushed, the enzyme myrosinase can help convert glucobrassicin into unstable intermediates that form I3C under suitable conditions. Commercial indole-3-carbinol powder ingredients may be obtained through specialized extraction or other manufacturing processes, so buyers should verify the actual source and production method with the supplier.

Q4. What is synthetic indole-3-carbinol made from?

Synthetic indole-3-carbinol is manufactured through controlled chemical reactions using appropriate starting materials and processing steps. The specific synthesis route can vary by manufacturer. For bulk I3C procurement, buyers should review the manufacturing process, residual solvents, related substances, and other potential impurities. A documented specification and validated analytical methods help determine whether the material indole-3-carbinol powder is suitable for the intended application.

Q5. How can manufacturers identify natural versus synthetic I3C?

Manufacturers should not rely on appearance alone to distinguish natural I3C from synthetic indole-3-carbinol powder because both may appear as white to off-white powders. Routine analytical techniques, such as HPLC, can help confirm identity and assess purity, but they may not independently establish the ingredient's origin. Buyers should request source declarations, manufacturing information, traceability records, and supporting documentation. Additional origin-verification testing may be necessary when required.

Q6. Are the purity requirements for natural and synthetic I3C different?

Purity requirements depend on the intended use, applicable regulations, and the purchaser's quality specifications rather than the ingredient's origin alone. Both natural and synthetic I3C should be evaluated for identity, assay, related substances, residual solvents where relevant, and potential contaminants. For supplement manufacturing, buyers should establish acceptance criteria and confirm that each batch complies with the agreed Certificate of Analysis (COA).

Q7. Is natural I3C more stable than synthetic indole-3-carbinol?

Natural origin does not necessarily make indole-3-carbinol powder more stable. The stability of natural and synthetic I3C depends on the chemical composition, purity, processing history, moisture, temperature, light exposure, and packaging conditions. I3C can also undergo chemical changes under acidic conditions. Manufacturers should request stability data, conduct compatibility studies, and establish appropriate storage conditions for their specific formulation.

Q8. What should supplement formulators consider when choosing natural or synthetic I3C?

Formulators should evaluate several technical and commercial factors before selecting an indole-3-carbinol powder ingredient:

- Source and manufacturing method: Confirm whether the ingredient is plant-derived or chemically synthesized.

- Purity and identity: Review validated analytical results.

- Impurity profile: Assess related substances, residual solvents, and relevant contaminants.

- Stability: Evaluate sensitivity to temperature, moisture, light, and formulation conditions.

- Regulatory compliance: Confirm suitability for the target market and intended use.

- Documentation and traceability: Request COA, technical specifications, and relevant manufacturing records.

These criteria support consistent raw material qualification and formulation development.

Q9. Can natural and synthetic I3C be used in the same supplement formulations?

Both forms may be considered for supplement formulations, provided they meet applicable regulatory requirements and the manufacturer's specifications. However, formulators should not assume that different sources will have identical impurity profiles or stability characteristics. Compatibility, content uniformity, and finished-product stability should be evaluated using the actual ingredient grade selected for production.

 

Product Qualification

Indole-3-carbinol Bulk Powder is produced in a clean working environment and every step of the process is conducted by highly qualified professionals. Both the production processes of manufacture and the product itself meet all international standards. And if you need our testing report, we can provide you.

 

Our factory:

There are two factories both in Baoji city and Shangluo City, Guanjie factories covers an area of 7000㎡, including GMP standard workshop of 2000㎡.

 

Certification

We have obtained the NON-GMO by Pony test international group. And we get the certifications about HALAAL,ISO 9001,ISO 22000,Kosher, PAHS,SC and so on.

 

Package&Warehouse:

 

Shipment:

DHL, Sea freight, FEDEX, you can choose.

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