Gamma Aminobutyric Acid Powder

Introduction:

Gamma aminobutyric acid powder (GABA), a naturally occurring non-protein amino acid, is a very important inhibitory neurotransmitter in the mammalian central nervous system. Gamma-aminobutyric acid (GABA) is produced from L-glutamate under the catalysis of glutamate decarboxylase. In the central nervous system, when GABAergic neurons release GABA, it binds to the corresponding receptors on the synapses of adjacent neurons, preventing the neuron from generating action potentials and thus preventing the neuron from releasing its neurotransmitters. neurotransmitters within it, so that it cannot affect other nerve cells that come into contact with it.

There are at least three types of γ-aminobutyric acid receptors: ionotropic channel GABA-A, GABA-C receptors, and metabotropic GABA-B receptors. Among them, GABA-A and GABA-C are ligand-gated chloride ion channels. When GABA binds to them, the receptors open and allow chloride ions to flow into the neuron, making the cell membrane super-saturated, thereby reducing the possibility of action potential generation; GABA-B are metabotropic receptors that, when activated, cause the opening of potassium ion channels that allow positively charged potassium ions to flow out of the neuron, again supercharging the neuron and inhibiting nerve cell activity.

 

 

Why Choose GUANJIE As a GABA Bulk Powder Supplier?

● We can do the third-party test.

● We guarantee the quality is in conformity with the Certificate of Analysis provided by us; if not, we accept 100% exchange, return back and refund.

● can reply to you within one hour for any inquiry questions and complaints.

● We can do OEM/ODM, sachet package.

● Our products are certified with ISO 9001-22000, SC, KOSHER, HALAL.

 

COA

Product Name	Gamma-aminobutyric acid powder	Manufacture Date	Feb. 08, 2022
Batch Number	GA220208	Analysis Date	Feb. 13, 2022
Quantity	500kgs	Certificate Date	Feb. 07, 2025
Analysis	Specification	Results
Assay	> 98%	99.8%
Physical & Chemical Control
Identification	Positive	Complies
Appearance	Yellow or white, crystalline powder	Complies
Odor	Characteristic	Complies
Taste	Characteristic	Complies
Loss on drying	<0.5%	0.3%
Residual Solvents	Eur.Pharm.	Complies
Heavy Metals	<10PPM	0.40PPM
Pb	<2.0ppm	Complies
As	<2.0ppm	Complies
Hg	<0.5ppm	Complies
Cd	<0.5ppm	Complies
Pesticides	Negative	Negative
Microbiological
Total Plate Count	<1000CFU/gm	Complies
Yeast&Mold	<100CFU/gm	Complies
Salmonella	Negative	Negative
E.Coli.	Negative	Negative
Conclusion
Storage	Store in cool & dry place, Keep away from strong light and heat.
Shelf life	18 Months when properly stored

 

Test Method:

A.1 Principle

Gamma aminobutyric acid powder was quantitatively derivatized with o-phthalaldehyde to generate isoindole derivatives with strong fluorescence and UV absorption. Different amino acid derivatives entering the chromatographic column at the same time, due to the difference in dissolution, adsorption, permeation or ion exchange between the mobile phase and the stationary phase, are repeatedly distributed between the two phases of the chromatographic column with the mobile phase. Due to the different moving speeds of each component in the chromatographic column, after passing through a certain length of chromatographic column, they are separated from each other, flow out of the chromatographic column in sequence, enter the signal detector, and display each component on the recorder or data detection and processing device. The peak value of the spectrum was quantified by the external standard method according to the retention time.

A. 2 Instruments

A.2.1 High-performance liquid chromatograph equipped with UV or evaporative light diffraction detector and data processing system

A.2.2 Column: C18

A. 2. 3 Analytical balance: Sensitive amount 0.1 mg

A. 2.4 Ultrasonic dissolver

A. 2.5 Microporous filter

A. 2.6 Volumetric flask: 100 mL

A. 2. 7 Syringe Filter (0.22 μm)

A.3 Reagents:

Unless otherwise specified, all reagents used in the analysis were of analytical grade.

A.3.1 Methanol: Chromatographic Grade

A.3.2 acetonitrile: chromatographic grade

A.3.3 Orthophthalaldehyde (OPA)

A. 3.4 Triethylamine

A.3.5 Crystalline Sodium Acetate

A.3.6 Trichloroacetic acid

A.3.7 Glacial acetic acid

A.3.8 Tetrahydrofuran

A.3.9 Gamma-aminobutyric acid standard: the purity is not less than 99.0%

A.3.10 Boric acid

A.3.11 Sodium hydroxide

A.4 Analysis steps

A.4.1 Preparation of standard solutions

Accurately weigh 0.05g of gamma-aminobutyric acid standard (accurate to 0.0001 g), dissolve it with water and dilute to 100 mL, filter with a 0.22 μm membrane, and collect the filtrate as the standard solution.

A.4.2 Preparation of sample solutions

Accurately weigh 0.05 g ~ 0.25 g of the pulverized sample (accurate to 0.0001 g), dissolve it with water and dilute to 100 mL, after ultrasonic dissolving, filter it with a 0.22 μm filter membrane, and collect the filtrate as the sample solution to be tested.

A.4.3 Preparation of 0.4 mol/L boric acid buffer

Accurately weigh 2.47 g of boric acid (accurate to 0.000 1 g), add about 80 mL of water, adjust the pH to 10.2 with sodium hydroxide, and dilute to 100 mL with water

A.4.4 Preparation of derivatizing reagents

Weigh 0.1 g of o-phthalaldehyde (OPA), dissolve it in 1 mL of acetonitrile, add 130 μL of mercaptoethanol, and make up to 10 mL with 0.4 mol/L boric acid buffer

A.4.5 Pre-column derivatization

Precisely measure the specified volume of sample solution and 5.0 μL of derivatization reagent, mix and react for about 2 minutes before injecting

A.4.6 Chromatographic analysis conditions

A.4.6.1 Mobile Phase

Phase A: Weigh 8.0 g of crystalline sodium acetate, dissolve it in water and make up to 1 000 mL; then add 220 μL of triethylamine, stir and add 5% acetic acid dropwise to adjust the pH to 7.20+0.02; finally add 5 mL of tetrahydrobark N, mixed and filtered, set aside

Phase B: Weigh 8.0 g of crystalline sodium acetate, dissolve it in water and dilute to 1 000 mL; then add 2% acetic acid dropwise to adjust the pH to 7.20±0.02; then press sodium acetate solution: acetonitrile: methanol = 1:2: 2 (volume ratio) filter after mixing and set aside

The gradient elution procedure is shown in Table A.1

Time/min	A/%	B/%	Speed/ (mL/min)
0	92	8	1.0
20	60	40	1.0
24	0	100	1.5
24.5	0	100	1.5
26.5	100	0	1.0
28	92	8	1.0

A.4.6.2 Column temperature: 40 °C

A.4.6.3 Flow rate: 1.0 mL/min

A.4.6.4 Detection wavelength: 338 nm

A.4.7 Standard curve

Accurately draw 2 μL, 4 μL, 5 μL, 10 μL, 12 μL of standard solution (0.5 mg/mL), respectively, perform chromatographic analysis according to A.4.4 and A.4.5, and take the peak area - the injection volume of the standard solution as the Figure, draw the standard curve and regression equation, the linear correlation coefficient R should be above 0.999 0

A.4.8 Sample Determination

Take the prepared sample and measure it according to A.4.4. Record the retention time and peak area of the chromatographic peak, and the time from the derivatization of the sample and the standard solution to the injection should be consistent. According to the peak area of the chromatographic peak, the corresponding concentration of gamma-aminobutyric acid was calculated by the external standard method. The response value of the analyte in the sample solution should be within the linear range determined by the instrumen

 

Function

● Gamma-aminobutyric acid powder can promote the release of growth hormone from the pituitary gland; at the same time, gamma-aminobutyric acid can also reduce the probability of insomnia, prolong deep sleep time, and improve sleep quality. Adequate sleep time is also important for children to grow taller, because the human body The pulsatile secretion of growth hormone peaks at night, so appropriate gamma-aminobutyric acid supplementation has certain benefits for normal development and height in children.

 

● It can enter the tricarboxylic acid cycle in the brain, promote brain cell metabolism, and at the same time increase the activity of glucose phosphatase during glucose metabolism, increase the production of acetylcholine, dilate blood vessels to increase blood flow, reduce blood ammonia, promote Brain metabolism, and restore brain cell function.

 

● GABA powder can inhibit the decarboxylation of glutamic acid and reduce blood ammonia. More glutamic acid is combined with ammonia to generate urea and excreted to relieve ammonia toxicity, thereby improving liver function.

 

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Certification

 

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