Background of enzymatic synthesis of NMN
NMN is an important coenzyme precursor molecule involved in energy metabolism and anti-aging processes in the human body. With the deepening of scientific research, NMN is widely used in nutraceuticals and drugs. The traditional chemical synthesis method is not suitable for large-scale production due to the high temperature and high pressure and the generation of many by-products. Guanjie Biotech is an NMN powder supplier. we use enzymatic synthesis to produce NMN bulk powder.
Principle
The NMN all-enzyme method uses NMN bulk powder synthetase (NMNAT) to catalyze a reaction on NAD substrate, linking AMP and NMN molecules in the NAD substrate to form an NMN molecule. The chemical equation for the reaction is NAD+NMN↔NADP+H++NADPH+NMN. NMNAT is a catalase. It catalyzes the reaction in both forward and reverse directions. When the concentration of NAD substrate in the reaction system is higher than the concentration of NMN molecules, the reaction will proceed mainly in the direction of NMN synthesis.
Steps
•Preparation of the enzyme:
Firstly the gene encoding NMN synthase needs to be introduced into a suitable host cell by genetic engineering techniques. Such as E. coli, yeast, etc. Then the host cells are cultured by fermentation technique to express NMN synthase in large quantities. Afterward, the cells are crushed and purified to obtain highly pure NMN powder synthetase.
•Substrate preparation:
Suitable NAD substrates and other co-substrates, usually nicotinamide adenine dinucleotide (NAD+) or its reduced form (NADH), need to be selected and dissolved in a suitable buffer and adjusted to the appropriate concentration and pH.
•Catalytic reaction:
Mix the prepared NMN synthetase with the substrate solution in a specific reaction vessel and control the conditions such as reaction temperature, pH and reaction time. The enzyme catalyzes the reaction of the substrate to produce NMN, which requires constant stirring or oscillation to ensure that the reaction proceeds adequately.
•Separation and purification of products:
At the end of the reaction, the NMN in the reaction mixture needs to be separated from the enzyme, unreacted substrate and other impurities. The separation methods usually used are filtration, centrifugation, chromatography and crystallization. High-purity NMN bulk powder products can be obtained by these methods.
Advantages
NMN prepared by bio-all-enzyme technology has the following advantages.
•High purity and activity:
The enzyme is highly specific and selective, able to catalyze specific chemical reactions with precision and reduce the occurrence of side reactions. This results in higher purity and biological activity of the synthesized NMN. The purity of the product can be stably maintained above 99.9%. This can greatly improve the quality of the product.
•Low impurity and high safety:
Bio-all-enzyme technology mimics the metabolic pathway of NMN in vivo, with virtually no additional impurities. Compared with the chemical synthesis method, it avoids the impurities brought by the use of chemical solvents, etc., and reduces the risk of impurities accumulating in the body, so it is safer.
Production process
•Mild reaction conditions:
Enzyme-catalyzed reactions are usually carried out under milder conditions. For example, room temperature, normal pressure, and near-neutral pH value. This avoids the effects of high temperatures, high pressures, strong acids and bases on the product and the environment, and reduces energy consumption and equipment requirements. It also reduces problems such as product denaturation or degradation that may result from extreme conditions.
•Green:
Biological all-enzyme technology does not produce harmful substances and is less polluting to the environment. It is in line with the concept of green chemistry and sustainable development. Moreover, the bio-enzyme can be reused, which further reduces the production cost and waste of resources.
•Large-scale production can be realized:
With the continuous development of biotechnology, the production efficiency and stability of enzymes are improving, and the all-enzyme method technology is becoming more and more mature. Enterprises can optimize reaction conditions and equipment to achieve large-scale NMN bulk powder, continuous production, improve production efficiency and meet market demand.
•Compatibility with human physiological processes
The core steps of the all-enzyme technology completely mimic the pathway of natural NMN synthesis in the human body. The resulting NMN is more similar in structure and function to endogenous NMN in the human body. Therefore, after entering the human body, NMN can be better recognised and utilised by the cells, and is more compatible with the physiological needs of the human body. This can exert more effective physiological effects.
How To Judge NMN Use Biocomplete Enzyme?
Consumers can determine if an NMN bulk powder product uses Bio-Optimal Enzyme Technology in the following ways.
• Purity:
Bio-all-enzymatic techniques typically result in high purity of the synthesized NMN. The purity of NMN synthesized by bioprocessing is usually high and can be maintained above 99.9%. If the product has the purity test report from the authoritative institution and the purity reaches this level, the bio-all-enzyme technology may be used. Guanjie Biotech is an NMN bulk powder manufacturer. We have our own QA and QC center. If you need the third-party testing report. we can also provide.
• Chiral structure:
Bio-all-enzymatic techniques allow for the precise synthesis of biologically active β-NMN, whereas chemical synthesis often produces a mixture of α-NMN and β-NMN. If a product is tested for chiral structure and contains only high purity β-NMN, it is more likely to be bio-all-enzymatic.
• Impurity content:
Since the bio-all-enzyme method produces essentially no additional impurities, the impurities associated with the use of chemical solvents, etc., are avoided compared to chemical synthesis. Suppose a product's impurity content test report shows that it has very few impurities and meets the criteria for a high-quality NMN. In that case, this can also be used as circumstantial evidence of the use of bio-all-enzymatic technology.
• Strength:
Choose a manufacturer with production capability, Guanjie Biotech has a strong R&D team and scientific research capability, we have mastered and applied the technology of the bio-all-enzyme method. We have a strong R&D team and scientific research capability, and we have mastered and applied bioprocessing technology. We have strict control over every procedure of product quality and production process. High quality.
Our pure NMN powder products pass HACCP, HAHAL, KOSHER, ISO90001, and other patents. If you are a supplement manufacturer and want to add NMN bulk powder to your products, welcome to consider our products. Our Nicotinamide Mononucleotide powder is available in different bulk densities to suit various applications.
•0.15 g/ml: Ideal for solid drinks (freeze-drying process).
•0.55 g/ml: Suitable for filling size 0# and 00# capsules (vacuum drying process).
•0.75 g/ml: Suitable for filling size 1# and 2# capsules (vacuum drying process).
All variants maintain a purity of over 99.5%.
