Cholic Acid Powder

Cholic Acid Powder Manufacturer & Supplier | High-Purity Bulk Cholic Acid Ingredient

Guanjie Biotech specializes in bulk cholic acid powder research, producing and exporting for more than 20 years. Cholic Acid is the main component of bile acids. It is insoluble in water, soluble in ethanol and acetic acid, and solid as white crystals. We have our own factories and two production lines, so the quality can be guaranteed. Welcome to enquire with us if you are interested.

 

What is Guanjie Biotech's Cholic Acid Powder?

Guanjie Biotech is a leading manufacturer of high-purity Cholic Acid Powder (CAS 81-25-4, ≥98% purity). Extracted through advanced purification techniques, our cholic acid serves as a key raw material for pharmaceutical synthesis (such as Ursodeoxycholic Acid/UDCA), dietary supplements, and biochemical research. We provide full COA, MSDS, and global shipping.

 

COA:

Items	Specification	Result
Appearance	A white powder	White powder
Identification (by HPLC)	The retention time of the principal peak in the test solution HPLC chromatogram obtained under conditions described in section 'Related substances to the retention time of the principal peak in the standard solution.	Pass
Melting point	197℃-202℃	197.7℃-198.8℃
Loss on drying	≤1.0%	0.35%
Sulphated ash	≤0.20%	0.03%
Related substance(HPLC)	≥95%(Purity)	97.41%
	≤2.0%(Single highestimpurity)	0.98%
	≤5.0%(Totalimpurities)	2.59%
Assay (by titration)	98.0%~101.0%	98.7%
Conclusion	Confirm

 

Purity analysis (Related substances)-TLC Test

1. Standard: Lithocholic acid - NMT 0.1%

2. Experimental method:

• Spreading agent: chloroform: glacial acetic acid: pure water = 8:15:0.5 mixed.

• Sample preparation:

①Test solution 1: Accurately weigh the sample 0.40g Cholic Acid Powder, use solution A <1> solvent, and fix the volume to 10ml.(40mg/ml)

②Test solution 2: Take 1ml of test solution 1 and dilute to 10ml.(4mg/ml)

 

• Preparation of standard solution

①Standard solution 1 (confirm CDCA): Weigh 40 mg of CDCA standard, dissolve with solution A, and fix the volume to 10ml.(4mg/ml)

②Standard solution 2 (confirm LCA): Weigh 20mg of Lithophanic acid sample, dissolve with solution A, and fix the volume to 10ml. take 2ml of this solution in a 100ml volumetric flask, and fix the volume with solution A to the marker line. (40μg/ml)

 

• Experimental conditions

①Thin layer plate: silica gel G

②Color developer: phosphomolybdic acid: ethanol = 1:5

③Spot sample volume: 5μl

④Expansion: 16~18cm

 

• Experimental method

① Put the standard solution to be examined in order on the TLC plate, using 5μl spotting tube to spot the plate.

②Put into a chromatography cylinder filled with saturated vapor of unfolding agent for unfolding. (about 1.5h).

③Dry in an oven (about 120℃) after unfolding for about 30 minutes

④After spraying the color developer evenly onto the TLC plate, dry it in an oven (about 120℃) for about 2~3 minutes and analyze the results.

Solution A<1>: acetone: pure water = 9:1

 

3. Experimental results

Lithophanic acid: the point of the test solution was higher than the point of the standard (dark, light)

 

Purity analysis (Related substances Ⅱ)-liquid phase

1. Standard

● Individual related substances: NMT1.5% (Area%)

● Other total substances: NMT5.0% (Area%)

 

2. Experimental method

● Mobile phase: acetonitrile: buffer solution: methanol = 30:37:40

● Preparation of test solution: weigh 120mg of Cholic Acid Powder sample, dissolve with diluent solution, and volume to 20ml.

● Diluent solution: Methanol: mobile phase = 10:90

● Buffer solution: 0.78g of sodium dihydrogen phosphate dissolved in 1L of pure water; phosphoric acid can be adjusted pH value of 3.

● The same concentration should be maintained when the amount of mobile phase made is increased

● Experimental conditions

⑤Column: 4.6mm×250mm, 5μmC18

⑥Temperature: 40°C±1°C

⑦Detector: differential detector (RID) (35℃±1℃)

⑧ Flow rate: 0.8ml/min (CDCA retention time of about 42min)

⑨Injection volume: 75μl

⑩Analysis time: 60min

 

What Is Cholic Acid Powder Used For?

Cholic acid powder, a natural bile acid derived from animal sources, has various applications across different industries. With its unique properties and functions, it finds utility in pharmaceuticals, biochemical research, cell culture, cosmetics, and the food industry.

 

Pharmaceutical Industry:

Pharmaceutical API Intermediate: Acts as a foundational precursor for synthesizing therapeutic bile derivatives including Ursodeoxycholic Acid (UDCA), Obeticholic Acid, and Chenodeoxycholic Acid (CDCA) used in gallstone dissolution and liver disease therapies.

• Active Ingredient:

Purified cholic acid is the cornerstone treatment for specific inborn errors of bile acid synthesis, such as Cerebrotendinous Xanthomatosis (CTX). In patients with CTX, a genetic defect prevents the endogenous production of sufficient cholic acid, leading to the accumulation of toxic metabolic intermediates. Administering cholic acid powder CAS 81-25-4 wholesale as a medication (e.g., in capsules) provides exogenous replacement, bypassing the defective pathway, normalizing bile acid pools, and preventing severe neurological and cardiovascular complications. This represents a life-changing therapy for affected individuals.

• Formulation Excipient:

Beyond its therapeutic use, Primary bile acids and their salts (e.g., sodium cholate) are employed as natural solubilizing agents and penetration enhancers. They are incorporated into drug formulations to improve the bioavailability of poorly water-soluble active compounds. By forming micelles, cholic acid can encapsulate hydrophobic drugs, allowing them to be absorbed in the aqueous environment of the digestive tract, thereby increasing their efficacy.

 

Biochemical and microbiological research

In the research laboratory, Pharmaceutical grade / Food grade Cholic Acid is an indispensable reagent.

• Protein Solubilization:

Its detergent properties are harnessed to solubilize membrane proteins and lipids. Researchers use it to break open cell membranes and isolate integral membrane proteins for purification and study without denaturing them, which is essential for understanding their structure and function.

• Micelle Formation Studies:

As a classic bile acid, Bulk Cholic Acid powder 98% / 99% is used extensively in biophysical studies to understand the principles of micelle formation, critical for research in lipidomics and nanotechnology.

• Microbiological Media Supplement:

In bacteriology, specific bile salts, including cholic acid derivatives, are added to culture media as selective agents. They inhibit the growth of Gram-positive bacteria while allowing Gram-negative bacteria (like those in the Enterobacteriaceae family) to grow, which is vital for the identification of pathogens like Salmonella and E. coli.

 

Cell Culture

In advanced cell culture, particularly in stem cell research and maintaining specialized cell lines, cholic acid is used as a supplement in the culture media. It aids in the absorption of lipids and fat-soluble vitamins by the cells, mimicking the natural physiological environment more closely. This is especially important for hepatocyte (liver cell) cultures and organoid models, where proper lipid metabolism is crucial for maintaining cell health, functionality, and experimental accuracy.

 

Cosmetic and Personal Care Industry

The surfactant properties of cholic acid powder are leveraged in cosmetics and skincare.

• Emulsification:

It helps to create stable emulsions, ensuring that oil and water-based ingredients in lotions, creams, and serums do not separate.

• Penetration Enhancement:

Similar to its pharmaceutical application, it can improve the skin's absorption of other active ingredients in a formulation, such as vitamins or antioxidants, potentially increasing the product's effectiveness.

• Natural Origin:

As a compound derived from nature, it is often marketed as a biocompatible and natural alternative to synthetic surfactants.

 

Food Industry

While its use is more niche, cholic acid powder serves as a processing aid in the food industry.

• Emulsifier:

It can be used in minute quantities to emulsify fats during the production of certain foods, creating a uniform texture and preventing separation.

• Precursor for Synthetic Sweeteners:

Notably, it can serve as a starting material for the synthesis of certain high-intensity sweeteners.

 

Frequently Asked Questions (FAQ)

Q1: What is the purity grade and source of Guanjie Biotech's Cholic Acid Powder?

Our Cholic Acid Powder features a high assay purity of ≥98.0% or ≥99.0% (determined by HPLC). It is extracted and refined from natural bovine or porcine bile via an advanced, multi-step biological separation process. We ensure total batch-to-batch Cholic Acid Powder consistency and complete raw material traceability in strict compliance with global BSE/TSE requirements.

Q2: Which quality standards and regulatory certificates do you support?

Our manufacturing facilities operate under GMP, ISO9001, and ISO22000 quality systems. The product complies with international USP and EP monograph limits. Furthermore, we can provide HALAL and KOSHER certifications alongside standard regulatory documentation for smooth customs clearance and compliance audits.

Q3: What analytical and quality documents are included with each Cholic Acid purchase?

Every dispatched order includes:

• COA (Certificate of Analysis): Batch-specific assay, heavy metals, and physical property results.

• MSDS / SDS: Safety Data Sheet available in multiple languages.

• TDS & HPLC Chromatogram: Full technical spec sheets and purity spectrum prints.

• Third-Party Testing: Heavy metal (ICP-MS), residual solvent (GC), and microbiological test reports from certified laboratories (e.g., SGS/Eurofins) are provided upon request.

Q4: What are the primary commercial and research applications of Cholic Acid Powder?

Natural Cholic Acid (CAS 81-25-4) serves several critical industries:

• Pharmaceutical API Synthesis:

Precursor for synthesizing therapeutic bile derivatives, such as Ursodeoxycholic Acid (UDCA), Obeticholic Acid, and Chenodeoxycholic Acid (CDCA).

• Drug Formulation & Excipient:

Functions as a bio-surfactant to improve the solubility, stability, and membrane permeability of hydrophobic drugs.

• Biochemical Research & Culture Media:

Solubilizing agent for membrane protein extraction and a key surfactant in microbiological media.

• Nutritional & Animal Feed Additives:

Aids in lipid digestion, micelle formation, and fat-soluble vitamin absorption in specialized health and animal nutrition formulas.

Q5: Is Cholic Acid Powder soluble in water? What are its solubility characteristics?

Pure Cholic Acid is practically insoluble in water and dilute acids (such as 0.1 M HCl). However, it is freely soluble in organic solvents such as ethanol, methanol, glacial acetic acid, acetone, and DMSO, and sparingly soluble in dilute alkaline solutions (0.1 M NaOH). If water solubility is required for your formulation, we can provide technical guidance on salt conversion (e.g., Sodium Cholates).

Q6: Do you offer Cholic Acid free samples for client R&D testing?

Yes, Guanjie Biotech offers 10g to 50g free evaluation Cholic Acid Powder samples for prospective commercial clients to perform assay validation, HPLC profiling, and formulation testing. While the sample material is complimentary, the buyer is responsible for standard express freight charges (or providing an active FedEx/DHL account number).

 

Quality Control, Compliance & E-E-A-T Standards

At Guanjie Biotech, quality assurance is integrated into every step of raw material cholic acid Powder collection, extraction, crystallization, and testing.

• Full Certification Compliance:

Our production line operates under ISO9001, ISO22000, HALAL, and KOSHER certifications, adhering strictly to cGMP standards.

• Comprehensive Documentation Support:

Every shipment includes a Certificate of Analysis (COA), Safety Data Sheet (MSDS/SDS), Technical Data Sheet (TDS), and HPLC test spectrum. Third-party testing reports (e.g., Eurofins, SGS) are available upon request.

• Advanced Quality Control Testing:

Heavy metals, pesticide residues, microbial limits, and solvent residues are rigorously monitored using GC-MS and ICP-MS equipment.

 

Certification

 

Packaging, Storage & Logistics

Attribute	Details
Packaging Options	1kg / Aluminum Foil Bag (Sample/Small orders); 25kg / Paper Drum with double food-grade PE inner bags. Custom packaging available.
Minimum Order Quantity (MOQ)	1 kg
Sample Availability	Free samples (10g – 50g) available for client lab testing and R&D evaluation (shipping costs applied).
Storage Conditions	Store in tightly sealed containers in a cool, dry, well-ventilated area away from direct sunlight, moisture, and heat.
Shelf Life	24 months when properly stored.
Logistics & Delivery	Worldwide shipping via FedEx, DHL, air freight, or sea freight. Terms: FOB, CIF, DDP. Lead time: 3–5 working days upon order confirmation.

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