1. Food Industry
• Meat tenderisation
Principle: Bromelain can break down myogenic fibres and collagen in meat. Bromelain (2400 GDU active enzyme) is added to meat during processing. The enzyme acts on the proteins in the muscle fibres to loosen their structure. For example, in older cuts of beef or pork, these enzymes hydrolyse the Z-discs in the muscle and the collagen in the intermuscular connective tissue, giving the meat a juicier texture.
• Improve baked goods quality
Bromelain powder bulk improves the properties of dough in bakery products. It breaks down the gluten proteins in the dough, further weakening the gluten network structure. This has the advantage of increasing the ductility and softness of the dough, which facilitates the shaping of the dough and the texture after baking. For some bakery products that require fine modelling, the use of 2400GDU of Bromelain can help the dough to be rolled out and folded better. Such as Danish bread, lasagne, etc.
Bromelain is added to the flour at the flour mixing stage, and the amount added is usually calculated based on the weight of the flour. Usually, 1 - 5 g of bromelain is added per 100 kg of flour. During the mixing process, the enzyme is evenly distributed in the dough and exerts its hydrolytic effect on the gluten proteins.
• Beverage clarification
Pure bulk bromelain powder can be used for clarification in the production of beverages such as fruit juices and beer. Some protein components in beverages may cause turbidity. Bromelain can hydrolyse these proteins. This can turn them into smaller peptides and amino acids. This can reduce the cloudiness of the drink. For juices with high levels of pectin and protein, 2400GDU's Bromelain effectively breaks down these components. It can improve the clarity of beverages. Such as apple juice, pear juice, etc.
In the clarification process of beverage production, the organic bromelain powder solution is added to the beverage to be clarified. The amount of enzyme and the reaction time are determined according to the type of beverage, the protein content and the initial turbidity. Generally, at the right pH (usually 4 - 6) and temperature (20 - 30°C), a significant clarification effect can be observed within 1 - 2 hours.
2. Pharmaceutical field
• Anti-inflammatory and anti-oedema
Bromelain has anti-inflammatory properties. It can reduce inflammatory exudates by breaking down fibrin at the site of inflammation. This reduces swelling. In the treatment of inflammation following some traumatic injuries or surgeries, pure bromelain powder from 2400 GDU improves blood circulation and helps the body remove inflammatory mediators. For example, in the treatment of soft tissue contusions, Bromelain can inhibit the further development of the inflammatory response and accelerate the tissue repair process.
3. Beauty & Skin Care Area
• Exfoliation
The skin's stratum corneum is mainly made up of keratin, which can be broken down by Bromelain. For areas of the skin where the cuticle is thicker, the use of 2400GDU's pure bulk bromelain powder product can gently exfoliate the skin to make it smoother and more delicate. Such as elbows and knees. It works by hydrolysing the proteins in the stratum corneum to shed aged keratinocytes and promote the production of new cells.
We can add pineapple protease to skin care products such as cleansers, scrubs or exfoliating gels. When using these products, follow the normal cleansing or exfoliating steps and gently massage the skin. Allow the enzyme to come into full contact with the stratum corneum, and generally use 1 - 2 times a week to achieve better exfoliation results.
• Improve skin inflammation
Bromelain enzyme powder can reduce inflammation when it occurs in the skin. It works by breaking down proteins at the site of inflammation, reducing the build-up of inflammatory cells and the release of inflammatory mediators. This relieves symptoms such as redness, swelling and itching of the skin. For mild skin inflammation, Bromelain from 2400GDU can be used as a natural adjunctive treatment ingredient.
Apply skin care products (e.g. lotions, gels, etc.) containing Bromelain to inflamed skin areas. 1 - 2 times per day. During application, observe the skin's reaction. Avoid adverse reactions such as allergies.
What Factors Affect The Action Of Bromelain Enzymes?
1. Temperature factor
• Curvilinear relationship between activity and temperature
Bromelain activity and temperature show a certain curvilinear relationship. In the lower temperature range, the activity of the enzyme gradually increases as the temperature rises. The optimum temperature for pure bulk bromelain powder is usually around 37 - 40°C. The enzyme is active in the lower temperature range. In this temperature range, the active centre of the enzyme molecule binds to the substrate most efficiently. It can break down substrates, such as gelatin and proteins, quickly and efficiently.
• Effect of too high or too low temperature
When the temperature exceeds the optimum temperature, the activity of the enzyme decreases dramatically. This is because high temperatures cause the denaturation of enzyme proteins. Enzyme proteins have complex secondary and tertiary structures. These structures are essential for maintaining enzyme activity. Excessively high temperatures destroy these structures of the enzyme molecule, causing a conformational change in the active centre.
Conversely, at too low a temperature, although the structure of the enzyme is not destroyed, the thermal movement of the molecule becomes very slow, and the frequency of collisions between the enzyme and the substrate is greatly reduced, resulting in a slower reaction rate. However, the enzyme activity can usually be restored as soon as the temperature rises back to the appropriate range.
2. pH Factor
• General effect of pH on enzyme activity
Pure bulk bromelain powder activity is also strongly influenced by pH. Different pH environments will change the charge state of the active centre of the enzyme molecule and the conformation of the enzyme protein. Within a certain pH range, enzyme activity varies with pH. Generally speaking, pineapple protease shows better activity in the range of pH 6 - 8.
3. Substrate concentration factor
• Relationship between Mie equation and substrate concentration
According to Mie's equation, when the concentration of substrate is low, with the increase of substrate concentration, the opportunity for enzyme and substrate binding increases, and the reaction rate is accelerated. Bromelain extract powder also follows this law. When the concentration of substrate (such as protein) is low, increasing the substrate concentration can make the enzyme action more obvious.
4. Enzyme concentration factor
Under the condition of sufficient substrate, the concentration of bromelain is positively related to the reaction rate. In other words, increasing the enzyme concentration can speed up the reaction rate.
Bromelain 2400 is widely used in many fields of healthcare, food, pharmaceutical and cosmetics due to its potent proteolytic ability. It is widely used in many fields such as health care, food, pharmaceuticals and cosmetics. Guanjie Biotech is a trusted bulk bromelain powder supplier, offering premium 2400 GDU bromelain powder. Our pure bulk bromelain powder products are certified with HALAL, Kosher, HACCP, ISO9001, and other quality standards. If you're looking to buy bromelain powder, feel free to contact us at info@gybiotech.com.
