Carob Seed Extract Powder

Carob Seed Extract Powder Supplier:

Guanjie Biotech is a Carob Seed Extract Powder supplier. Our D－Pinitol powder is from natural carob beans that grow along the Mediterranean coast. We provide 95%-98% D－Pinitol. High quality and competitive price. Welcome to enquiry info@gybiotech.com.

COA:

Item	Specification	Result	Method
Basic Product Information
Country of Origin	China	Conform	/
Marker Compounds
D－Pinitol(on dried basis)	>98.0%	99.31%	HPLC
Organoleptic Data
Appearance	Powder	Conform	NLS-QCS-1008
Color	White powder	Conform	GB/T 5492-2008
Odor	Characteristic	Conform	GB/T 5492-2008
Taste	Characteristic	Conform	GB/T 5492-2008
Process Data
Method of Processing	Extraction	Conform	/
Solvent(s) Used	Ethanol & Water	Conform	/
Drying Method	Vacuum drying	Conform	/
Excipient	None	Conform	/
Physical Characteristics
Melting point	178-185°C	180-185°C	GB21781-2008T
Specific Optical Rotation	NLT﹢ 55°	﹢60.1°	GB 613-88
Particle Size (40 mesh)	98.0%pass 40mesh	Conform	GB/T 5507-2008
Loss on Drying	<1.5%	0.52%	GB/T 14769-1993
Ash Content	<1.5%	0.41%	AOAC 942.05, 18th
Solvent Residue	Ethanol	Conform	NLS-QCS-1007
Heavy Metals
Total Heavy Metals	<10 ppm	Conform	USP <231>, method II
Arsenic	<2.0 ppm	Conform	AOAC 986.15, 18th
Pesticide Residue
666	<0.2ppm	Conform	GB/T5009.19-1996
DDT	<0.2ppm	Conform	GB/T5009.19-1996
Microbiology
Total Plate Count	<1000/g	37/g	AOAC 990.12, 18th
Total Yeast & Mold	<100/g	<9/g	FDA (BAM) Chapter 18, 8th Ed.
E. Coli	Negative	Negative	AOAC 997.11, 18th
Salmonella	Negative	Negative	FDA (BAM) Chapter 5, 8th Ed.

 

Flow Chart：

Amicogen's carob seed extract powder D-Pinitol is manufactured according to Good Manufacturing Practice (GMP) approved by the Korea Food and Drug /Administration.

Extraction of carob bean pod with water

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Hydrolysis of the extract

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Filtration and vacuum concentration

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Separation with a charcoal column

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Vacuum concentration

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De-salting with ion exchanges

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Membrane filtration and vacuum concentration

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Crystallization

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Drying and Grinding

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Packaging

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D-Pinitol

Pinitol content analysis

1) machinery : HPLC(High performance liquid chromatography)

2) Preparation of Carob Seed Extract Powder Standard solution: Make the final volume 100 by adding 50% Acetonitrile of carob seed extract D-pinitol powder standard 1g. The standard solvent is to filter this by a 0.45 μm membrane filter.

3) Test solvent: Test solvent is made like standard solvent making.

4) Analysis

HPLC condition

Articles	Conditions
Column	Waters carbohydrate cartridge(4.6x250mm)
detector	RI
Column Tem.	40℃
Mobile phase	Water : Acetonitrile (15:85)
Flow	0.8ml/min
Injection vol.	20ul

(1) Quantitative analysis

Measure the width of the peak after 20ul injection of the test and standard solvents

each, calculate the carob seed extract powder pinitol content(%) with the below method.

Pinitol content(%)= (B/A) * purity of standard one(%)

A : Area value of the standard solvent

B : Area value of test solvent

To measure more accurate contents, repeat the above analysis 3~5 times to decide the final

pinitol content with the average value.

 

Moisture content analysis method

1) Heat at 105'C for 4 hrs in a dry oven and measure the weighing balance of the room temperature (a).

2) Measure accurate 3g Carob Seed Extract Powder of subject on regular weighing balance (b).

3) Dry at 105'c for 4 hrs in a dry oven

4) Place at room temperature for 30 mins at desiccator after drying

5) Measure the regular weighing balance(c).

6) Test result

Moisture (%)= b-c *100

                       b-a

a: Weight of weighing balance(g)

b: Weight of weighing balance and subject(g)

c: Weigh after drying(g)

 

What is D-pinitol?

Carob Seed Extract Powder ​​​​​​is a naturally occurring substance that belongs to the class of inositols, a class of compounds consisting of nine different isomers. Inositol is similar to a six-membered monosaccharide (i.e. glucose). However, it is not a sugar, but a cycloalditol. Like monosaccharides, inositol is a part of the human diet. And there is evidence that it is non-toxic. Pineol differs from other inositols in that it contains a methyl ether group. Studies have shown that pinitol has different biological activities than other inositols. The natural product pinitol is found in certain legumes (such as soybeans), some other plants, fruits, and pine trees. Chemically, carob seed extract powder D-pinitol is defined as inositol. The best source of pinitol is in carob pods. Carob trees are resistant to adverse environmental conditions and generally do not require special treatments such as intensive farming or pesticides.

 

Properties:

Carob Seed Extract Powder D-Pinitol is a sugar alcohol compound widely found in plants. The following are its specific chemical and physical properties.

1. Chemical properties

• Structural characteristics:

D-Pinitol Carob Seed Extract Powder belongs to the cyclohexane polyol compound. Its molecular structure is five hydroxyl groups and one methyl group connected to cyclohexane. It has a specific stereochemical structure. It has unique chemical properties and biological activity.

• Stability:

Carob Seed Extract Powder D-Pinitol is relatively stable under normal environmental conditions. However, under extreme conditions such as high temperature, strong acid, and strong base, its molecular structure may change. For example, under strong acid conditions, protonation of hydroxyl groups may occur, resulting in changes in the activity and properties of the molecule. When heated at high temperatures for a long time, this may cause a dehydration reaction to generate products containing double bonds, etc.

• Reactivity

The multiple hydroxyl groups in the molecule make D-Pinitol have the typical reactivity of alcohols. It can undergo an esterification reaction with an acid to generate corresponding ester compounds. For example, reaction with acetic acid can generate D-Pinitol acetate.

The hydroxyl groups of D-Pinitol can be oxidized. For example, under the action of appropriate oxidants, hydroxyl groups can be oxidized to carbonyl or carboxyl groups. This will generate oxidation products with different functions and properties.

Hydroxyl groups can also participate in etherification reactions. It reacts with other compounds containing hydroxyl groups or halogen atoms to form ether compounds.

 

2. Physical properties

• Appearance:

Carob Seed Extract Powder is usually a white crystalline powder with a delicate appearance. Under a microscope, its crystals have a certain shape and a regular lattice structure.

• Odor and taste:

Carob Seed Extract Powder has no obvious odor, with a light sweetness. This sweetness is relatively mild. It is not as sweet as common sugars such as sucrose.

• Solubility:

Carob Seed D-pinitol is easily soluble in water and can quickly dissolve in water to form a clear solution. This is because the multiple hydroxyl groups in its molecules can form hydrogen bonds with water molecules, thereby promoting the dissolution process. It also has good solubility in some polar organic solvents such as methanol and ethanol. But it has poor solubility in non-polar organic solvents such as petroleum ether and benzene.

• Melting point

The melting point of Carob Seed Extract Powder bulk D-pinitol is generally between 178-182℃. When the melting point is reached, D-pinitol changes from solid to liquid. This temperature range is the characteristic temperature of its solid-liquid transition and can be used for its purity identification and physical property research.

 

Factory&Team：

 

Certification

 

Package & Shipment

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