Oleuropein Powder

Guanjie Biotech is an oleuropein powder supplier. Our bulk oleuropein is a yellow-green to light-yellow powder with an assay from 10% to 80%. We use the ethanol/water extraction methods.  And the particle size is 98% through 80 mesh. It is a type of polyphenolic compound derived from the leaves of olive trees, specifically related to olive leaf extract. It is a bitter-tasting, dark yellow powder made from the leaves of Olea europaea (olive) trees. Our Oleuropein Powder products are high-quality and competitive price. Welcome to enquire with us at info@gybiotech.com.

● We can do the third-party test.

● We can reply to you within one hour for any inquiries, questions, and complaints about Oleuropein Powder.

● The company has a 3,000-acre olive planting base in Longnan, Gansu. We control the quality and stability of raw materials from the source. From the entry of raw materials to the final product, we strictly control in accordance with GMP requirements to ensure product quality, and the annual processing of raw materials can reach 600 tons.
● The company has advanced imported testing equipment. This can ensure traceability testing at every link from raw materials to production.
● Professional technical team, strictly control quality and quality.
● The Oleuropein Powder has low pesticide residue, low solvent residue and high purity.

● Our products are certified with ISO 9001-22000, SC, KOSHER, HALAL.

 

Item		Specifications	Test Method
Oleuropein content%		10%-80%	HPLC
Physics and Chemistry
Color		Yellow-green or light yellow fine powder	Visual inspection
Odor and taste		Oleuropein special smell and taste	Sensory
Particle size		98.0%pass 80mesh	CP2015 Edition Four General Rules 0982
Moisture (105℃, 3H)		≤5.0%	GB 5009.3-2016
Ash		≤5.0%	GB 5009.4-2016
Bulk density	(compactness)	65g/100ml	USP36 616
	(bulk density)	95g/100ml	USP36 616
Process Data
Method of Processing		Extraction	Conform
Solvent(s) Used		Ethanol& Water	Conform
Heavy Metals
As		≤2.0ppm	GB 5009.11-2014
Pb		≤2.0ppm	GB 5009.12-2017
Cd		≤1.0ppm	GB 5009.15-2014
Hg		≤0.1ppm	GB 5009.17-2014
Microbiology
Total bacteria count		≤1000cfu/g	GB 4789.2-2016
Total mold and yeast count		≤100cfu/g	GB 4789.15-2016
Escherichia coli		Not to be detected	GB 4789.3-2016
Salmonella		Not to be detected	GB 4789.4-2016
Staphylococcus aureus		Not to be detected	GB 4789.10-2016

High-Performance Liquid Chromatography (HPLC) is the most widely used method for the identification and quantification of oleuropein due to its high sensitivity, specificity, and reproducibility. Guanjie Biotech can test each batch of Oleuropein Powder.

●Sample Preparation
To analyze oleuropein powder using HPLC, an appropriate sample preparation procedure is followed. Typically, a known amount of oleuropein powder is accurately weighed and dissolved in a solvent such as methanol, ethanol, or a methanol-water mixture. The solution is then subjected to sonication to enhance dissolution and subsequently filtered through a 0.45 μm membrane filter to remove particulate matter before injection into the HPLC system.

●Chromatographic Conditions
The HPLC analysis is generally performed using a reverse-phase C18 column. This type of column effectively retains the moderately polar oleuropein molecule. A commonly used mobile phase consists of a mixture of water (often acidified with phosphoric acid or acetic acid) and acetonitrile or methanol in gradient or isocratic elution mode. For example, a typical mobile phase might be 0.1% phosphoric acid in water (solvent A) and acetonitrile (solvent B) with a flow rate of 1.0 mL/min.

The column temperature is typically maintained at 25–30°C, and the UV detection wavelength is set around 280 nm, which corresponds to the maximum absorbance of oleuropein Powder. The injection volume is usually 10–20 µL.

●Quantification
Quantification of oleuropein is achieved by preparing a calibration curve using oleuropein Powder reference standards at different concentrations. The peak area of the sample is compared to that of the standards, allowing accurate determination of oleuropein content in the powder. Retention time for oleuropein typically ranges from 10 to 20 minutes, depending on the exact chromatographic conditions.

 

Oleuropein Powder is produced using a variety of methods, including extraction, chemical synthesis, and fermentation. Extraction is the most common method used to produce oleuropein powder. This process involves the use of high-pressure liquid chromatography (HPLC) and its derivatives. Through these processes, oleuropein is extracted from the herb and isolated from other plant compounds. The extracted oleuropein is then processed and dried into a fine powder. Guanjie Biotech uses ethanol/water extraction. Let's look at its production process. Two common extraction solvents used in its production are ethanol and water, both offering distinct advantages depending on the desired purity, safety, and application.

●Ethanol Extraction Method
Oleuropein Powder Ethanol extraction is widely used in the production of oleuropein due to its efficiency in dissolving both polar and non-polar compounds. This method typically involves drying and grinding olive leaves or fruit, followed by soaking or refluxing the plant material in ethanol or an ethanol–water mixture under controlled temperatures. Ethanol concentrations may vary between 50% and 90%, depending on the target compound concentration.
One of the key advantages of ethanol extraction is its ability to yield a high-purity oleuropein extract with good bioactivity. Ethanol is also a food-grade solvent, generally recognized as safe (GRAS), which makes it suitable for supplements and food applications. After extraction, the mixture is usually filtered, concentrated under reduced pressure, and spray-dried to obtain fine oleuropein powder.

●Water Extraction Method
Water extraction is a more environmentally friendly and cost-effective method, especially appealing for organic and clean-label products. In this process, the olive leaves are boiled or soaked in water at controlled temperatures, usually below 80°C, to extract oleuropein. Since oleuropein is moderately soluble in water, longer extraction times or the use of enzymes (such as cellulase or pectinase) may be required to enhance yield.
Although water extraction is less efficient compared to ethanol, it offers a safe and non-toxic alternative for Oleuropein Powder applications where solvent residues are a concern. It also aligns with the principles of green chemistry, making it favorable for natural health products and eco-friendly formulations.

Both ethanol and water extraction methods are effective for obtaining oleuropein powder, and the choice largely depends on the desired purity, regulatory requirements, and end-use application. Ethanol extraction offers higher efficiency and concentration, while water extraction prioritizes safety and sustainability. In commercial production, a combination of both solvents is sometimes used to optimize yield and maintain a balance between efficiency and environmental responsibility.

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