Luteolin Powder of Guanjie Biotech stands out in the market due to its advanced technological application in controlling ash content, a significant factor influenced by the physical properties of luteolin. Traditionally, the market has seen luteolin powders with ash content around 10%. High ash content can indicate lower purity and effectiveness of the flavonoid. We have pioneered a technological solution to significantly reduce the ash content in their luteolin powder. We reduce the ash content from the typical 10% to under 2%.
Luteolin is a naturally occurring flavonoid. Its chemical name is 3', 4', 5, 7-tetrahydroxyflavone. It its widely distributed in the plant kingdom. For example, it is present in acacia, peanut shells, honeysuckle, wild chrysanthemum and broccoli. Luteolin powder can be extracted from honeysuckle. The chemical structure formula is shown in Figure 1.
Luteolin has a high penetration capacity in the skin. It can reach deep into the skin layer. The effects are as follows: antioxidant, delayed aging, anti-inflammatory, whitening, antibacterial, inhibition and elimination of skin discoloration. However, it suffers from the important problem of poor solubility, which leads to the limitation of Luteolin powder application in cosmetics. Its solubility and utilization can be improved by solid dispersions, cyclodextrin inclusion complexes, phospholipid complexes, nanoemulsions, polymer micelles and other formulation techniques. In turn, it will work well. Figure 2 lists the efficacy studies of lignans related to cosmetics.
Advances in Formulation Research
01 Solid dispersion
Solid dispersion (SD) is a solid dispersion obtained by combining a refractory ingredient with a highly soluble carrier by fusion (fusion) method, solvent method or molten solvent method. This results in drug release and greatly increases the dissolution of insoluble drugs. The solubility of luteolin powder in luteolin solid dispersion (Lut-SD) and luteolin phospholipid complex solid dispersion (Lut-PC-SD) prepared by Xiangtao Deng et al. was increased from (61.09±0.09) µg/mL to 6-7 times of the original. The solubility comparison is shown in Figure 3.
02 Cyclodextrin inclusion complexes
Cyclodextrin is a cyclic oligosaccharide. It has a lipophilic inner cavity and a hydrophilic outer surface, and is able to interact with a variety of guest molecules to form non-covalent inclusion complexes. It is mainly used to enhance the water solubility, physicochemical stability and bioavailability of drugs. Xudong Wang et al. prepared sustained-release antimicrobial nanofibers (PVA-Lut-IC-NF) by incorporating luteolin powder inclusion complex (Lut-IC) into poly(vinyl alcohol) (PVA) by electrostatic spinning.PVA-Lut-IC-NF has good water solubility and thermal stability. And it has significant inhibitory effect on Staphylococcus aureus, Candida albicans and Escherichia coli.
03 Phospholipid complexes
Phospholipid complex is a self-assembly of liposome-like vesicles formed in water by interactions between phospholipids and active ingredients through non-covalent bonding (charge transfer, hydrogen bonding, van der Waals forces, etc.). It increases the stability of the active and prolongs the duration of action. Related studies have shown that phospholipid complexes containing lignans. The solubility in water is about 2.5 times that of pure luteolin powder.
04 Nanoemulsion
Nanoemulsion is made of two immiscible liquids. With the help of appropriate surfactant and co-surfactant composition of the particle size between 1 ~ 100 nm homogeneous dispersion system. It has the advantages of increasing the solubility of the active substance and improving the bioavailability, etc. Shin et al. prepared luteolin nanoemulsions consisting of poly(ethylene oxide)-block poly(ε-caprolactone) and lecithin polymerized at the O/W interface. It showed excellent stability during repeated freeze-thaw cycles and long-term storage. And minoxidil was used as a positive control to apply it to the depilated skin of mice, and the results showed similar hair growth after 17 days. However, the hair on the surface of the skin treated with luteolin powder nanoemulsion was more uniform and soft.
05 Polymeric micelles
Polymer micelles are amphiphilic block copolymers that self-assemble in water into ordered aggregates with a core/shell structure. The hydrophobic chains of the block copolymer form the core of the micelle. They are used to encapsulate hydrophobic substances. The hydrophilic chains form its outer shell and maintain the spatial stability of the micelle. It was shown that luteolin powder was assembled into methoxy polyethylene glycol polycaprolactone (mPEG5K-PCL10K) micelles. It not only has a high stability and encapsulation rate, but the water solubility of luteolin is also greatly improved.
Luteolin powder of Guanjie Biotech, with its low ash content technology, provides a superior option for researchers and industries looking for high quality luteolin. If you are intersted, welcome to enquiry us: info@gybiotech.com.
References:
[1] Wang JX. Introduction of cosmetic natural ingredient raw materials (ⅹⅪ)[J]. Science of Daily Chemicals,2021,44(02):56-60.
[2] Zhan X, Xu F, Zhu J et al. Progress of physiological effects and formulation of luteolin[J]. Daily Chemical Industry(In English and Chinese),2023,53(04):437-444.
[3] Pasarkar N, Waghmare S, Kamble H. Solid dispersion: a review ［J］. Iconic Research and Engineering Journals, 2022, 5(7): 6
[4] Deng Xiangtao, Hao Navy, Chen Xiaofeng, et al. Preparation, characterization and in vivo pharmacokinetic behavior of two solid dispersions of luteolin in rats[J]. Chinese Herbal Medicine,2018,49(24):5787-5793.
[5]Shin K, Choi H, Song S K, et al. Nanoemulsion vehicles as carriers for follicular delivery of luteolin ［J］. ACS Biomaterials Science & Engineering, 2018, 4(5): 1723-1729.
[6] Wang X, Hu X, Li S, et al. Preparation of antibacterial nanofibers by electrospinning polyvinyl alcohol containing a luteolin hydroxypropylβ-cyclodextrin inclusion complex ［J］. New Journal of Chemistry, The Royal Society of Chemistry, 2022, 46(5): 2360-2367.
